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Putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features
PURPOSE: In mice, adipose tissue-derived stem cells (ASCs) reach the systemic circulation and establish ectopic adipose depots fostering insulin resistance, but whether this occurs in humans is unknown. We examined circulating ASCs in individuals with various combination of metabolic syndrome traits...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514150/ https://www.ncbi.nlm.nih.gov/pubmed/36952215 http://dx.doi.org/10.1007/s40618-023-02067-7 |
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author | Bonora, B. M. Cappellari, R. Albiero, M. Prevedello, L. Foletto, M. Vettor, R. Avogaro, A. Fadini, G. P. |
author_facet | Bonora, B. M. Cappellari, R. Albiero, M. Prevedello, L. Foletto, M. Vettor, R. Avogaro, A. Fadini, G. P. |
author_sort | Bonora, B. M. |
collection | PubMed |
description | PURPOSE: In mice, adipose tissue-derived stem cells (ASCs) reach the systemic circulation and establish ectopic adipose depots fostering insulin resistance, but whether this occurs in humans is unknown. We examined circulating ASCs in individuals with various combination of metabolic syndrome traits. METHODS: We enrolled patients attending a routine metabolic evaluation or scheduled for bariatric surgery. We quantified ASCs as CD34(+)CD45(−)CD31(−)(CD36(+)) cells in the stromal vascular fraction of subcutaneous and visceral adipose tissue samples and examined the presence and frequency of putative ASCs in peripheral blood. RESULTS: We included 111 patients (mean age 59 years, 55% males), 40 of whom were scheduled for bariatric surgery. The population of CD34(+)CD45(−)CD31(−) ASCs was significantly more frequent in visceral than subcutaneous adipose depots (10.4 vs 4.1% of the stromal vascular fraction; p < 0.001), but not correlated with BMI or metabolic syndrome traits. The same phenotype of ASCs was detectable in peripheral blood of 58.6% of patients. Those with detectable circulating ASCs had significantly higher BMI (37.8 vs 33.3 kg/m(2); p = 0.003) and waist (111.2 vs 105.4 cm; p = 0.001), but no difference in other metabolic syndrome traits (p = 0.84). After bariatric surgery, patients with detectable circulating ASCs had greater BMI reductions at 6 months (− 10.4 vs − 7.8 kg/m(2); p = 0.014). CONCLUSION: Presence of putative circulating ASCs, antigenically similar to those observed in the adipose tissue, is associated with greater adiposity and larger BMI reduction after surgery, but not with clinical signs of metabolic impairment. The role of circulating ASCs in adipose tissue biology and systemic metabolism deserves further investigation. |
format | Online Article Text |
id | pubmed-10514150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-105141502023-09-23 Putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features Bonora, B. M. Cappellari, R. Albiero, M. Prevedello, L. Foletto, M. Vettor, R. Avogaro, A. Fadini, G. P. J Endocrinol Invest Original Article PURPOSE: In mice, adipose tissue-derived stem cells (ASCs) reach the systemic circulation and establish ectopic adipose depots fostering insulin resistance, but whether this occurs in humans is unknown. We examined circulating ASCs in individuals with various combination of metabolic syndrome traits. METHODS: We enrolled patients attending a routine metabolic evaluation or scheduled for bariatric surgery. We quantified ASCs as CD34(+)CD45(−)CD31(−)(CD36(+)) cells in the stromal vascular fraction of subcutaneous and visceral adipose tissue samples and examined the presence and frequency of putative ASCs in peripheral blood. RESULTS: We included 111 patients (mean age 59 years, 55% males), 40 of whom were scheduled for bariatric surgery. The population of CD34(+)CD45(−)CD31(−) ASCs was significantly more frequent in visceral than subcutaneous adipose depots (10.4 vs 4.1% of the stromal vascular fraction; p < 0.001), but not correlated with BMI or metabolic syndrome traits. The same phenotype of ASCs was detectable in peripheral blood of 58.6% of patients. Those with detectable circulating ASCs had significantly higher BMI (37.8 vs 33.3 kg/m(2); p = 0.003) and waist (111.2 vs 105.4 cm; p = 0.001), but no difference in other metabolic syndrome traits (p = 0.84). After bariatric surgery, patients with detectable circulating ASCs had greater BMI reductions at 6 months (− 10.4 vs − 7.8 kg/m(2); p = 0.014). CONCLUSION: Presence of putative circulating ASCs, antigenically similar to those observed in the adipose tissue, is associated with greater adiposity and larger BMI reduction after surgery, but not with clinical signs of metabolic impairment. The role of circulating ASCs in adipose tissue biology and systemic metabolism deserves further investigation. Springer International Publishing 2023-03-23 2023 /pmc/articles/PMC10514150/ /pubmed/36952215 http://dx.doi.org/10.1007/s40618-023-02067-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Bonora, B. M. Cappellari, R. Albiero, M. Prevedello, L. Foletto, M. Vettor, R. Avogaro, A. Fadini, G. P. Putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features |
title | Putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features |
title_full | Putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features |
title_fullStr | Putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features |
title_full_unstemmed | Putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features |
title_short | Putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features |
title_sort | putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514150/ https://www.ncbi.nlm.nih.gov/pubmed/36952215 http://dx.doi.org/10.1007/s40618-023-02067-7 |
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