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Xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast
mRNA degradation is one of the main steps of gene expression, and a key player is the 5’-3’ exonuclease Xrn1. In Saccharomyces cerevisiae , it was previously shown, by a microscopy approach, that Xrn1 is located to different cellular compartments, depending on physiological state. During exponential...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514700/ https://www.ncbi.nlm.nih.gov/pubmed/37746059 http://dx.doi.org/10.17912/micropub.biology.000926 |
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author | Courtin, Baptiste Namane, Abdelkader Gomard, Maite Meyer, Laura Jacquier, Alain Fromont-Racine, Micheline |
author_facet | Courtin, Baptiste Namane, Abdelkader Gomard, Maite Meyer, Laura Jacquier, Alain Fromont-Racine, Micheline |
author_sort | Courtin, Baptiste |
collection | PubMed |
description | mRNA degradation is one of the main steps of gene expression, and a key player is the 5’-3’ exonuclease Xrn1. In Saccharomyces cerevisiae , it was previously shown, by a microscopy approach, that Xrn1 is located to different cellular compartments, depending on physiological state. During exponential growth, Xrn1 is distributed in the cytoplasm, while it co-localizes with eisosomes after the post-diauxic shift (PDS). Here, we biochemically characterize the Xrn1-associated complexes in different cellular states. We demonstrate that, after PDS, Xrn1 but not the decapping nor Lsm1-7/Pat1 complexes associates with eisosomal proteins, strengthening the model that sequestration of Xrn1 in eisosomes preserves mRNAs from degradation during PDS. |
format | Online Article Text |
id | pubmed-10514700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-105147002023-09-23 Xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast Courtin, Baptiste Namane, Abdelkader Gomard, Maite Meyer, Laura Jacquier, Alain Fromont-Racine, Micheline MicroPubl Biol Findings Previously Not Shown mRNA degradation is one of the main steps of gene expression, and a key player is the 5’-3’ exonuclease Xrn1. In Saccharomyces cerevisiae , it was previously shown, by a microscopy approach, that Xrn1 is located to different cellular compartments, depending on physiological state. During exponential growth, Xrn1 is distributed in the cytoplasm, while it co-localizes with eisosomes after the post-diauxic shift (PDS). Here, we biochemically characterize the Xrn1-associated complexes in different cellular states. We demonstrate that, after PDS, Xrn1 but not the decapping nor Lsm1-7/Pat1 complexes associates with eisosomal proteins, strengthening the model that sequestration of Xrn1 in eisosomes preserves mRNAs from degradation during PDS. Caltech Library 2023-09-07 /pmc/articles/PMC10514700/ /pubmed/37746059 http://dx.doi.org/10.17912/micropub.biology.000926 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Findings Previously Not Shown Courtin, Baptiste Namane, Abdelkader Gomard, Maite Meyer, Laura Jacquier, Alain Fromont-Racine, Micheline Xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast |
title | Xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast |
title_full | Xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast |
title_fullStr | Xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast |
title_full_unstemmed | Xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast |
title_short | Xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast |
title_sort | xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast |
topic | Findings Previously Not Shown |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514700/ https://www.ncbi.nlm.nih.gov/pubmed/37746059 http://dx.doi.org/10.17912/micropub.biology.000926 |
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