Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis

Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cu...

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Detalles Bibliográficos
Autores principales: Michaliski, Lamonielli F., Ióca, Laura P., Oliveira, Leandro S., Crnkovic, Camila M., Takaki, Mirelle, Freire, Vitor F., Berlinck, Roberto G. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514814/
https://www.ncbi.nlm.nih.gov/pubmed/37736960
http://dx.doi.org/10.3390/mps6050077
Descripción
Sumario:Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cultivation parameters, followed by chemometrics analysis on HPLC-UV-MS or UHPLC-HRMS/MS data, is presented to enhance the production yield of fungal natural products. The overall procedure typically requires 3–4 months of work when first developed, and up to 3 months as a routine procedure.

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