Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis

Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cu...

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Autores principales: Michaliski, Lamonielli F., Ióca, Laura P., Oliveira, Leandro S., Crnkovic, Camila M., Takaki, Mirelle, Freire, Vitor F., Berlinck, Roberto G. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514814/
https://www.ncbi.nlm.nih.gov/pubmed/37736960
http://dx.doi.org/10.3390/mps6050077
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author Michaliski, Lamonielli F.
Ióca, Laura P.
Oliveira, Leandro S.
Crnkovic, Camila M.
Takaki, Mirelle
Freire, Vitor F.
Berlinck, Roberto G. S.
author_facet Michaliski, Lamonielli F.
Ióca, Laura P.
Oliveira, Leandro S.
Crnkovic, Camila M.
Takaki, Mirelle
Freire, Vitor F.
Berlinck, Roberto G. S.
author_sort Michaliski, Lamonielli F.
collection PubMed
description Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cultivation parameters, followed by chemometrics analysis on HPLC-UV-MS or UHPLC-HRMS/MS data, is presented to enhance the production yield of fungal natural products. The overall procedure typically requires 3–4 months of work when first developed, and up to 3 months as a routine procedure.
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spelling pubmed-105148142023-09-23 Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis Michaliski, Lamonielli F. Ióca, Laura P. Oliveira, Leandro S. Crnkovic, Camila M. Takaki, Mirelle Freire, Vitor F. Berlinck, Roberto G. S. Methods Protoc Protocol Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cultivation parameters, followed by chemometrics analysis on HPLC-UV-MS or UHPLC-HRMS/MS data, is presented to enhance the production yield of fungal natural products. The overall procedure typically requires 3–4 months of work when first developed, and up to 3 months as a routine procedure. MDPI 2023-08-29 /pmc/articles/PMC10514814/ /pubmed/37736960 http://dx.doi.org/10.3390/mps6050077 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Michaliski, Lamonielli F.
Ióca, Laura P.
Oliveira, Leandro S.
Crnkovic, Camila M.
Takaki, Mirelle
Freire, Vitor F.
Berlinck, Roberto G. S.
Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis
title Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis
title_full Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis
title_fullStr Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis
title_full_unstemmed Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis
title_short Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis
title_sort improvement of targeted fungi secondary metabolite production using a systematic experimental design and chemometrics analysis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514814/
https://www.ncbi.nlm.nih.gov/pubmed/37736960
http://dx.doi.org/10.3390/mps6050077
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