Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation

Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the efficient replication of ASFV for these purposes. This should be...

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Autores principales: Puzankova, Olga, Gavrilova, Vera, Chernyshev, Roman, Kolbin, Ivan, Igolkin, Alexey, Sprygin, Alexandr, Chvala, Ilya, Mazloum, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514816/
https://www.ncbi.nlm.nih.gov/pubmed/37736956
http://dx.doi.org/10.3390/mps6050073
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author Puzankova, Olga
Gavrilova, Vera
Chernyshev, Roman
Kolbin, Ivan
Igolkin, Alexey
Sprygin, Alexandr
Chvala, Ilya
Mazloum, Ali
author_facet Puzankova, Olga
Gavrilova, Vera
Chernyshev, Roman
Kolbin, Ivan
Igolkin, Alexey
Sprygin, Alexandr
Chvala, Ilya
Mazloum, Ali
author_sort Puzankova, Olga
collection PubMed
description Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the efficient replication of ASFV for these purposes. This should be achieved even when starting with a low virus load, as in the case of isolating the virus from field samples. This article presents a detailed protocol on the preparation of porcine bone marrow primary (PBMP) cell culture, which has a high sensitivity towards ASFV, resulting in high viral yields with a minimal risk of bacterial contamination.
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spelling pubmed-105148162023-09-23 Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation Puzankova, Olga Gavrilova, Vera Chernyshev, Roman Kolbin, Ivan Igolkin, Alexey Sprygin, Alexandr Chvala, Ilya Mazloum, Ali Methods Protoc Protocol Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the efficient replication of ASFV for these purposes. This should be achieved even when starting with a low virus load, as in the case of isolating the virus from field samples. This article presents a detailed protocol on the preparation of porcine bone marrow primary (PBMP) cell culture, which has a high sensitivity towards ASFV, resulting in high viral yields with a minimal risk of bacterial contamination. MDPI 2023-08-24 /pmc/articles/PMC10514816/ /pubmed/37736956 http://dx.doi.org/10.3390/mps6050073 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Puzankova, Olga
Gavrilova, Vera
Chernyshev, Roman
Kolbin, Ivan
Igolkin, Alexey
Sprygin, Alexandr
Chvala, Ilya
Mazloum, Ali
Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation
title Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation
title_full Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation
title_fullStr Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation
title_full_unstemmed Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation
title_short Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation
title_sort novel protocol for the preparation of porcine bone marrow primary cell culture for african swine fever virus isolation
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514816/
https://www.ncbi.nlm.nih.gov/pubmed/37736956
http://dx.doi.org/10.3390/mps6050073
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