Cargando…
Utilizing Dichloromethane as an Extremely Proficient Substitute for Phenol/Chloroform in Extracting RNA with Exceptional Purity from Woody Tissues of Coconut
Procuring high-grade RNA from mature coconut tissues is a tricky and labor-intensive process due to the intricate scaffold of polysaccharides, polyphenols, lipids, and proteins that form firm complexes with nucleic acids. However, we have effectively developed a novel method for the first time, lett...
Autores principales: | Iqbal, Amjad, Yang, Yaodong |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514881/ https://www.ncbi.nlm.nih.gov/pubmed/37736958 http://dx.doi.org/10.3390/mps6050075 |
Ejemplares similares
-
To Ascertain the Purity of Chloroform
Publicado: (1862) -
A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing
por: Barbier, François F., et al.
Publicado: (2019) -
QRREM method for the isolation of high-quality RNA from the complex matrices of coconut
por: Iqbal, Amjad, et al.
Publicado: (2019) -
Chloroform—a Substitute for Ether
Publicado: (1847) -
Optimization of phenol-chloroform RNA extraction
por: Toni, Lee S., et al.
Publicado: (2018)