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Fruit size control by a zinc finger protein regulating pericarp cell size in tomato
Fruit size is largely defined by the number and size of cells in the fruit. Endoreduplication – a specialized cell cycle – is highly associated with cell expansion during tomato fruit growth. However, how endoreduplication coupled with cell size is regulated remains poorly understood. In this study,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10515234/ https://www.ncbi.nlm.nih.gov/pubmed/37789485 http://dx.doi.org/10.1186/s43897-021-00009-6 |
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author | Zhao, Fangfang Zhang, Jiajing Weng, Lin Li, Meng Wang, Quanhua Xiao, Han |
author_facet | Zhao, Fangfang Zhang, Jiajing Weng, Lin Li, Meng Wang, Quanhua Xiao, Han |
author_sort | Zhao, Fangfang |
collection | PubMed |
description | Fruit size is largely defined by the number and size of cells in the fruit. Endoreduplication – a specialized cell cycle – is highly associated with cell expansion during tomato fruit growth. However, how endoreduplication coupled with cell size is regulated remains poorly understood. In this study, we identified a zinc finger gene SlPZF1 (Solanum lycopersicum PERICARP-ASSOCIATED ZINC FINGER PROTEIN 1) that was highly expressed in the pericarp of developing fruits. Plants with altered SlPZF1 expression produced smaller fruits due to the reduction in cell size associated with weakened endoreduplication. Overexpressing SlPZF1 delayed cell division phase by enhancing early expression of several key cell cycle regulators including SlCYCD3;1 and two plant specific mitotic cyclin-dependent protein kinase (SlCDKB1 and SlCDKB2) in the pericarp tissue. Furthermore, we identified 14 putative SlPZF1 interacting proteins (PZFIs) via yeast two hybrid screening. Several PZFIs, including Pre-mRNA-splicing factor (SlSMP1/PZFI4), PAPA-1-like conserved region family protein (PZFI6), Fanconi anemia complex components (PZFI3 and PZFI10) and bHLH transcription factor LONESOME HIGHWAY (SlLHW/PZFI14), are putatively involved in cell cycle regulation. Our results demonstrate that fruit growth in tomato requires balanced expression of the novel cell size regulator SlPZF1. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43897-021-00009-6. |
format | Online Article Text |
id | pubmed-10515234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-105152342023-09-27 Fruit size control by a zinc finger protein regulating pericarp cell size in tomato Zhao, Fangfang Zhang, Jiajing Weng, Lin Li, Meng Wang, Quanhua Xiao, Han Mol Hortic Research Article Fruit size is largely defined by the number and size of cells in the fruit. Endoreduplication – a specialized cell cycle – is highly associated with cell expansion during tomato fruit growth. However, how endoreduplication coupled with cell size is regulated remains poorly understood. In this study, we identified a zinc finger gene SlPZF1 (Solanum lycopersicum PERICARP-ASSOCIATED ZINC FINGER PROTEIN 1) that was highly expressed in the pericarp of developing fruits. Plants with altered SlPZF1 expression produced smaller fruits due to the reduction in cell size associated with weakened endoreduplication. Overexpressing SlPZF1 delayed cell division phase by enhancing early expression of several key cell cycle regulators including SlCYCD3;1 and two plant specific mitotic cyclin-dependent protein kinase (SlCDKB1 and SlCDKB2) in the pericarp tissue. Furthermore, we identified 14 putative SlPZF1 interacting proteins (PZFIs) via yeast two hybrid screening. Several PZFIs, including Pre-mRNA-splicing factor (SlSMP1/PZFI4), PAPA-1-like conserved region family protein (PZFI6), Fanconi anemia complex components (PZFI3 and PZFI10) and bHLH transcription factor LONESOME HIGHWAY (SlLHW/PZFI14), are putatively involved in cell cycle regulation. Our results demonstrate that fruit growth in tomato requires balanced expression of the novel cell size regulator SlPZF1. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43897-021-00009-6. BioMed Central 2021-08-14 /pmc/articles/PMC10515234/ /pubmed/37789485 http://dx.doi.org/10.1186/s43897-021-00009-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Zhao, Fangfang Zhang, Jiajing Weng, Lin Li, Meng Wang, Quanhua Xiao, Han Fruit size control by a zinc finger protein regulating pericarp cell size in tomato |
title | Fruit size control by a zinc finger protein regulating pericarp cell size in tomato |
title_full | Fruit size control by a zinc finger protein regulating pericarp cell size in tomato |
title_fullStr | Fruit size control by a zinc finger protein regulating pericarp cell size in tomato |
title_full_unstemmed | Fruit size control by a zinc finger protein regulating pericarp cell size in tomato |
title_short | Fruit size control by a zinc finger protein regulating pericarp cell size in tomato |
title_sort | fruit size control by a zinc finger protein regulating pericarp cell size in tomato |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10515234/ https://www.ncbi.nlm.nih.gov/pubmed/37789485 http://dx.doi.org/10.1186/s43897-021-00009-6 |
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