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Plasmids pick a bacterial partner before committing to conjugation
Bacterial conjugation was first described by Lederberg and Tatum in the 1940s following the discovery of the F plasmid. During conjugation a plasmid is transferred unidirectionally from one bacterium (the donor) to another (the recipient), in a contact-dependent manner. Conjugation has been regarded...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516633/ https://www.ncbi.nlm.nih.gov/pubmed/37592747 http://dx.doi.org/10.1093/nar/gkad678 |
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author | Frankel, Gad David, Sophia Low, Wen Wen Seddon, Chloe Wong, Joshua L C Beis, Konstantinos |
author_facet | Frankel, Gad David, Sophia Low, Wen Wen Seddon, Chloe Wong, Joshua L C Beis, Konstantinos |
author_sort | Frankel, Gad |
collection | PubMed |
description | Bacterial conjugation was first described by Lederberg and Tatum in the 1940s following the discovery of the F plasmid. During conjugation a plasmid is transferred unidirectionally from one bacterium (the donor) to another (the recipient), in a contact-dependent manner. Conjugation has been regarded as a promiscuous mechanism of DNA transfer, with host range determined by the recipient downstream of plasmid transfer. However, recent data have shown that F-like plasmids, akin to tailed Caudovirales bacteriophages, can pick their host bacteria prior to transfer by expressing one of at least four structurally distinct isoforms of the outer membrane protein TraN, which has evolved to function as a highly sensitive sensor on the donor cell surface. The TraN sensor appears to pick bacterial hosts by binding compatible outer membrane proteins in the recipient. The TraN variants can be divided into specialist and generalist sensors, conferring narrow and broad plasmid host range, respectively. In this review we discuss recent advances in our understanding of the function of the TraN sensor at the donor-recipient interface, used by F-like plasmids to select bacterial hosts within polymicrobial communities prior to DNA transfer. |
format | Online Article Text |
id | pubmed-10516633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105166332023-09-23 Plasmids pick a bacterial partner before committing to conjugation Frankel, Gad David, Sophia Low, Wen Wen Seddon, Chloe Wong, Joshua L C Beis, Konstantinos Nucleic Acids Res Critical Reviews and Perspectives Bacterial conjugation was first described by Lederberg and Tatum in the 1940s following the discovery of the F plasmid. During conjugation a plasmid is transferred unidirectionally from one bacterium (the donor) to another (the recipient), in a contact-dependent manner. Conjugation has been regarded as a promiscuous mechanism of DNA transfer, with host range determined by the recipient downstream of plasmid transfer. However, recent data have shown that F-like plasmids, akin to tailed Caudovirales bacteriophages, can pick their host bacteria prior to transfer by expressing one of at least four structurally distinct isoforms of the outer membrane protein TraN, which has evolved to function as a highly sensitive sensor on the donor cell surface. The TraN sensor appears to pick bacterial hosts by binding compatible outer membrane proteins in the recipient. The TraN variants can be divided into specialist and generalist sensors, conferring narrow and broad plasmid host range, respectively. In this review we discuss recent advances in our understanding of the function of the TraN sensor at the donor-recipient interface, used by F-like plasmids to select bacterial hosts within polymicrobial communities prior to DNA transfer. Oxford University Press 2023-08-18 /pmc/articles/PMC10516633/ /pubmed/37592747 http://dx.doi.org/10.1093/nar/gkad678 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Critical Reviews and Perspectives Frankel, Gad David, Sophia Low, Wen Wen Seddon, Chloe Wong, Joshua L C Beis, Konstantinos Plasmids pick a bacterial partner before committing to conjugation |
title | Plasmids pick a bacterial partner before committing to conjugation |
title_full | Plasmids pick a bacterial partner before committing to conjugation |
title_fullStr | Plasmids pick a bacterial partner before committing to conjugation |
title_full_unstemmed | Plasmids pick a bacterial partner before committing to conjugation |
title_short | Plasmids pick a bacterial partner before committing to conjugation |
title_sort | plasmids pick a bacterial partner before committing to conjugation |
topic | Critical Reviews and Perspectives |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516633/ https://www.ncbi.nlm.nih.gov/pubmed/37592747 http://dx.doi.org/10.1093/nar/gkad678 |
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