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The effects of BPA-BNCT on normal bone: determination of the CBE value in mice

Boron neutron capture therapy (BNCT) with p-boronophenylalanine (BPA) is expected to have less effect on the decrease in normal bone strength than X-ray therapy. However, the compound biological effectiveness (CBE) value necessary to convert the boron neutron capture reaction (BNCR) dose into a bioe...

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Autores principales: Iwasaki, Ryota, Yoshikawa, Ryutaro, Umeno, Ryo, Seki, Azusa, Matsukawa, Takehisa, Takeno, Satoshi, Yokoyama, Kazuhito, Mori, Takashi, Suzuki, Minoru, Ono, Koji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516729/
https://www.ncbi.nlm.nih.gov/pubmed/37517393
http://dx.doi.org/10.1093/jrr/rrad054
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author Iwasaki, Ryota
Yoshikawa, Ryutaro
Umeno, Ryo
Seki, Azusa
Matsukawa, Takehisa
Takeno, Satoshi
Yokoyama, Kazuhito
Mori, Takashi
Suzuki, Minoru
Ono, Koji
author_facet Iwasaki, Ryota
Yoshikawa, Ryutaro
Umeno, Ryo
Seki, Azusa
Matsukawa, Takehisa
Takeno, Satoshi
Yokoyama, Kazuhito
Mori, Takashi
Suzuki, Minoru
Ono, Koji
author_sort Iwasaki, Ryota
collection PubMed
description Boron neutron capture therapy (BNCT) with p-boronophenylalanine (BPA) is expected to have less effect on the decrease in normal bone strength than X-ray therapy. However, the compound biological effectiveness (CBE) value necessary to convert the boron neutron capture reaction (BNCR) dose into a bioequivalent X-ray dose has not been determined yet. The purpose of this study was to evaluate the influence of BNCT on normal bone in mice and to elucidate the CBE factor. We first searched the distribution of BPA in the normal bone of C3H/He mice and then measured the changes in bone strength after irradiation. The CBE value was determined when the decrease in bone strength was set as an index of the BNCT effect. The (10)B concentrations in the tibia after subcutaneous injection of 125, 250 and 500 mg/kg BPA were measured by prompt gamma-ray spectroscopy and inductively coupled plasma (ICP)-atomic emission spectrometry. The (10)B mapping in the tibia was examined by alpha-track autoradiography and laser ablation-ICP-mass spectrometry. The (10)B concentration increased dose-dependently; moreover, the concentrations were maintained until 120 min after BPA administration. The administered (10)B in the tibia was abundantly accumulated in the growth cartilage, trabecular bone and bone marrow. The bone strength was analyzed by a three-point bending test 12 weeks after irradiation. The bending strength of the tibia decreased dose-dependently after the irradiation of X-ray, neutron and BNCR. The CBE factor was obtained as 2.27 by comparing these dose-effect curves; the value determined in this study will enable an accurate dosimetry of normal bone.
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spelling pubmed-105167292023-09-24 The effects of BPA-BNCT on normal bone: determination of the CBE value in mice Iwasaki, Ryota Yoshikawa, Ryutaro Umeno, Ryo Seki, Azusa Matsukawa, Takehisa Takeno, Satoshi Yokoyama, Kazuhito Mori, Takashi Suzuki, Minoru Ono, Koji J Radiat Res Regular paper Boron neutron capture therapy (BNCT) with p-boronophenylalanine (BPA) is expected to have less effect on the decrease in normal bone strength than X-ray therapy. However, the compound biological effectiveness (CBE) value necessary to convert the boron neutron capture reaction (BNCR) dose into a bioequivalent X-ray dose has not been determined yet. The purpose of this study was to evaluate the influence of BNCT on normal bone in mice and to elucidate the CBE factor. We first searched the distribution of BPA in the normal bone of C3H/He mice and then measured the changes in bone strength after irradiation. The CBE value was determined when the decrease in bone strength was set as an index of the BNCT effect. The (10)B concentrations in the tibia after subcutaneous injection of 125, 250 and 500 mg/kg BPA were measured by prompt gamma-ray spectroscopy and inductively coupled plasma (ICP)-atomic emission spectrometry. The (10)B mapping in the tibia was examined by alpha-track autoradiography and laser ablation-ICP-mass spectrometry. The (10)B concentration increased dose-dependently; moreover, the concentrations were maintained until 120 min after BPA administration. The administered (10)B in the tibia was abundantly accumulated in the growth cartilage, trabecular bone and bone marrow. The bone strength was analyzed by a three-point bending test 12 weeks after irradiation. The bending strength of the tibia decreased dose-dependently after the irradiation of X-ray, neutron and BNCR. The CBE factor was obtained as 2.27 by comparing these dose-effect curves; the value determined in this study will enable an accurate dosimetry of normal bone. Oxford University Press 2023-07-29 /pmc/articles/PMC10516729/ /pubmed/37517393 http://dx.doi.org/10.1093/jrr/rrad054 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular paper
Iwasaki, Ryota
Yoshikawa, Ryutaro
Umeno, Ryo
Seki, Azusa
Matsukawa, Takehisa
Takeno, Satoshi
Yokoyama, Kazuhito
Mori, Takashi
Suzuki, Minoru
Ono, Koji
The effects of BPA-BNCT on normal bone: determination of the CBE value in mice
title The effects of BPA-BNCT on normal bone: determination of the CBE value in mice
title_full The effects of BPA-BNCT on normal bone: determination of the CBE value in mice
title_fullStr The effects of BPA-BNCT on normal bone: determination of the CBE value in mice
title_full_unstemmed The effects of BPA-BNCT on normal bone: determination of the CBE value in mice
title_short The effects of BPA-BNCT on normal bone: determination of the CBE value in mice
title_sort effects of bpa-bnct on normal bone: determination of the cbe value in mice
topic Regular paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516729/
https://www.ncbi.nlm.nih.gov/pubmed/37517393
http://dx.doi.org/10.1093/jrr/rrad054
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