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Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor

The development of genetically encoded dopamine sensors such as dLight has provided a new approach to measuring slow and fast dopamine dynamics both in brain slices and in vivo, possibly enabling dopamine measurements in areas like the dorsolateral striatum (DLS) where previously such recordings wit...

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Autores principales: Salinas, Armando G., Lee, Jeong Oen, Augustin, Shana M., Zhang, Shiliang, Patriarchi, Tommaso, Tian, Lin, Morales, Marisela, Mateo, Yolanda, Lovinger, David M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517008/
https://www.ncbi.nlm.nih.gov/pubmed/37739964
http://dx.doi.org/10.1038/s41467-023-41581-3
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author Salinas, Armando G.
Lee, Jeong Oen
Augustin, Shana M.
Zhang, Shiliang
Patriarchi, Tommaso
Tian, Lin
Morales, Marisela
Mateo, Yolanda
Lovinger, David M.
author_facet Salinas, Armando G.
Lee, Jeong Oen
Augustin, Shana M.
Zhang, Shiliang
Patriarchi, Tommaso
Tian, Lin
Morales, Marisela
Mateo, Yolanda
Lovinger, David M.
author_sort Salinas, Armando G.
collection PubMed
description The development of genetically encoded dopamine sensors such as dLight has provided a new approach to measuring slow and fast dopamine dynamics both in brain slices and in vivo, possibly enabling dopamine measurements in areas like the dorsolateral striatum (DLS) where previously such recordings with fast-scan cyclic voltammetry (FSCV) were difficult. To test this, we first evaluated dLight photometry in mouse brain slices with simultaneous FSCV and found that both techniques yielded comparable results, but notable differences in responses to dopamine transporter inhibitors, including cocaine. We then used in vivo fiber photometry with dLight in mice to examine responses to cocaine in DLS. We also compared dopamine responses during Pavlovian conditioning across the striatum. We show that dopamine increases were readily detectable in DLS and describe transient dopamine kinetics, as well as slowly developing signals during conditioning. Overall, our findings indicate that dLight photometry is well suited to measuring dopamine dynamics in DLS.
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spelling pubmed-105170082023-09-24 Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor Salinas, Armando G. Lee, Jeong Oen Augustin, Shana M. Zhang, Shiliang Patriarchi, Tommaso Tian, Lin Morales, Marisela Mateo, Yolanda Lovinger, David M. Nat Commun Article The development of genetically encoded dopamine sensors such as dLight has provided a new approach to measuring slow and fast dopamine dynamics both in brain slices and in vivo, possibly enabling dopamine measurements in areas like the dorsolateral striatum (DLS) where previously such recordings with fast-scan cyclic voltammetry (FSCV) were difficult. To test this, we first evaluated dLight photometry in mouse brain slices with simultaneous FSCV and found that both techniques yielded comparable results, but notable differences in responses to dopamine transporter inhibitors, including cocaine. We then used in vivo fiber photometry with dLight in mice to examine responses to cocaine in DLS. We also compared dopamine responses during Pavlovian conditioning across the striatum. We show that dopamine increases were readily detectable in DLS and describe transient dopamine kinetics, as well as slowly developing signals during conditioning. Overall, our findings indicate that dLight photometry is well suited to measuring dopamine dynamics in DLS. Nature Publishing Group UK 2023-09-22 /pmc/articles/PMC10517008/ /pubmed/37739964 http://dx.doi.org/10.1038/s41467-023-41581-3 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Salinas, Armando G.
Lee, Jeong Oen
Augustin, Shana M.
Zhang, Shiliang
Patriarchi, Tommaso
Tian, Lin
Morales, Marisela
Mateo, Yolanda
Lovinger, David M.
Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor
title Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor
title_full Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor
title_fullStr Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor
title_full_unstemmed Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor
title_short Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor
title_sort distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517008/
https://www.ncbi.nlm.nih.gov/pubmed/37739964
http://dx.doi.org/10.1038/s41467-023-41581-3
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