Structural and functional analysis of hyper-thermostable ancestral L-amino acid oxidase that can convert Trp derivatives to D-forms by chemoenzymatic reaction

Production of D-amino acids (D-AAs) on a large-scale enables to provide precursors of peptide therapeutics. In this study, we designed a novel L-amino acid oxidase, HTAncLAAO2, by ancestral sequence reconstruction, exhibiting high thermostability and long-term stability. The crystal structure of HTA...

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Detalles Bibliográficos
Autores principales: Kawamura, Yui, Ishida, Chiharu, Miyata, Ryo, Miyata, Azusa, Hayashi, Seiichiro, Fujinami, Daisuke, Ito, Sohei, Nakano, Shogo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517122/
https://www.ncbi.nlm.nih.gov/pubmed/37737277
http://dx.doi.org/10.1038/s42004-023-01005-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517122/
https://www.ncbi.nlm.nih.gov/pubmed/37737277
http://dx.doi.org/10.1038/s42004-023-01005-1

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