Identification of regulatory links between transcription and RNA processing with long-read sequencing

We present a detailed protocol for sequencing full-length mRNA isoforms using the Oxford nanopore long-read sequencing technology. We describe steps for poly(A) RNA isolation, library preparation, and cDNA size selection. We then detail procedures for sequencing and processing and a computational fr...

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Detalles Bibliográficos
Autores principales: Alfonso-Gonzalez, Carlos, Arrigoni, Laura, Ozbulut, Hasan Can, Falk, Stefanie, Bönisch, Ulrike, Hilgers, Valérie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517278/
https://www.ncbi.nlm.nih.gov/pubmed/37733595
http://dx.doi.org/10.1016/j.xpro.2023.102505

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