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Application of Single Extracellular Vesicle Analysis Techniques

Extracellular vesicles (EVs) are lipid containers that are actively released by cells and contain complex molecular cargoes. These cargoes include abundant material such as genomes and proteins from cells of origin. They are involved in intercellular communication and various pathological processes,...

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Detalles Bibliográficos
Autores principales: Zhu, Junquan, Wu, Feifeng, Li, Cuifang, Mao, Jueyi, Wang, Yang, Zhou, Xin, Xie, Haotian, Wen, Chuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518151/
https://www.ncbi.nlm.nih.gov/pubmed/37750091
http://dx.doi.org/10.2147/IJN.S421342
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author Zhu, Junquan
Wu, Feifeng
Li, Cuifang
Mao, Jueyi
Wang, Yang
Zhou, Xin
Xie, Haotian
Wen, Chuan
author_facet Zhu, Junquan
Wu, Feifeng
Li, Cuifang
Mao, Jueyi
Wang, Yang
Zhou, Xin
Xie, Haotian
Wen, Chuan
author_sort Zhu, Junquan
collection PubMed
description Extracellular vesicles (EVs) are lipid containers that are actively released by cells and contain complex molecular cargoes. These cargoes include abundant material such as genomes and proteins from cells of origin. They are involved in intercellular communication and various pathological processes, showing excellent potential for diagnosing and treating diseases. Given the significant heterogeneity of EVs in complex physiopathological processes, unveiling their composition is essential to understanding their function. Bulk detection methods have been previously used to analyze EVs, but they often mask their heterogeneity, leading to the loss of valuable information. To overcome this limitation, single extracellular vesicle (SEV) analysis techniques have been developed and advanced. These techniques allow for analyzing EVs’ physical information and biometric molecules at the SEV level. This paper reviews recent advances in SEV detection methods and summarizes some clinical applications for SEV detection strategies.
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spelling pubmed-105181512023-09-25 Application of Single Extracellular Vesicle Analysis Techniques Zhu, Junquan Wu, Feifeng Li, Cuifang Mao, Jueyi Wang, Yang Zhou, Xin Xie, Haotian Wen, Chuan Int J Nanomedicine Review Extracellular vesicles (EVs) are lipid containers that are actively released by cells and contain complex molecular cargoes. These cargoes include abundant material such as genomes and proteins from cells of origin. They are involved in intercellular communication and various pathological processes, showing excellent potential for diagnosing and treating diseases. Given the significant heterogeneity of EVs in complex physiopathological processes, unveiling their composition is essential to understanding their function. Bulk detection methods have been previously used to analyze EVs, but they often mask their heterogeneity, leading to the loss of valuable information. To overcome this limitation, single extracellular vesicle (SEV) analysis techniques have been developed and advanced. These techniques allow for analyzing EVs’ physical information and biometric molecules at the SEV level. This paper reviews recent advances in SEV detection methods and summarizes some clinical applications for SEV detection strategies. Dove 2023-09-20 /pmc/articles/PMC10518151/ /pubmed/37750091 http://dx.doi.org/10.2147/IJN.S421342 Text en © 2023 Zhu et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Review
Zhu, Junquan
Wu, Feifeng
Li, Cuifang
Mao, Jueyi
Wang, Yang
Zhou, Xin
Xie, Haotian
Wen, Chuan
Application of Single Extracellular Vesicle Analysis Techniques
title Application of Single Extracellular Vesicle Analysis Techniques
title_full Application of Single Extracellular Vesicle Analysis Techniques
title_fullStr Application of Single Extracellular Vesicle Analysis Techniques
title_full_unstemmed Application of Single Extracellular Vesicle Analysis Techniques
title_short Application of Single Extracellular Vesicle Analysis Techniques
title_sort application of single extracellular vesicle analysis techniques
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518151/
https://www.ncbi.nlm.nih.gov/pubmed/37750091
http://dx.doi.org/10.2147/IJN.S421342
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