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Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets
The field of stem cell-based embryo-like models is rapidly evolving, providing in vitro models of in utero stages of mammalian development. Here, we detail steps to first establish adherent spheroids composed of three cell types from mouse embryonic stem cells solely treated with a chemical inhibito...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518363/ https://www.ncbi.nlm.nih.gov/pubmed/37721864 http://dx.doi.org/10.1016/j.xpro.2023.102573 |
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author | Traboulsi, Tatiana Sart, Sébastien Baroud, Charles N. Dejean, Anne Cossec, Jack-Christophe |
author_facet | Traboulsi, Tatiana Sart, Sébastien Baroud, Charles N. Dejean, Anne Cossec, Jack-Christophe |
author_sort | Traboulsi, Tatiana |
collection | PubMed |
description | The field of stem cell-based embryo-like models is rapidly evolving, providing in vitro models of in utero stages of mammalian development. Here, we detail steps to first establish adherent spheroids composed of three cell types from mouse embryonic stem cells solely treated with a chemical inhibitor of SUMOylation. We then describe procedures for generating highly reproducible gastruloids from these dissociated spheroid cells, as well as embryo-like structures comprising anterior neural and trunk somite-like regions using an optimized microfluidics platform. For complete details on the use and execution of this protocol, please refer to Cossec et al. (2023).(1) |
format | Online Article Text |
id | pubmed-10518363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105183632023-09-26 Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets Traboulsi, Tatiana Sart, Sébastien Baroud, Charles N. Dejean, Anne Cossec, Jack-Christophe STAR Protoc Protocol The field of stem cell-based embryo-like models is rapidly evolving, providing in vitro models of in utero stages of mammalian development. Here, we detail steps to first establish adherent spheroids composed of three cell types from mouse embryonic stem cells solely treated with a chemical inhibitor of SUMOylation. We then describe procedures for generating highly reproducible gastruloids from these dissociated spheroid cells, as well as embryo-like structures comprising anterior neural and trunk somite-like regions using an optimized microfluidics platform. For complete details on the use and execution of this protocol, please refer to Cossec et al. (2023).(1) Elsevier 2023-09-17 /pmc/articles/PMC10518363/ /pubmed/37721864 http://dx.doi.org/10.1016/j.xpro.2023.102573 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Traboulsi, Tatiana Sart, Sébastien Baroud, Charles N. Dejean, Anne Cossec, Jack-Christophe Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets |
title | Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets |
title_full | Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets |
title_fullStr | Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets |
title_full_unstemmed | Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets |
title_short | Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets |
title_sort | generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of sumoylation and cultured in microdroplets |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518363/ https://www.ncbi.nlm.nih.gov/pubmed/37721864 http://dx.doi.org/10.1016/j.xpro.2023.102573 |
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