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A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy
INTRODUCTION: Sonodynamic therapy (SDT) has potential clinical applications for cancer therapy, and is yet restricted by complex tumor microenvironmental (TME) factors. Thus, the research problem of TME modulation as well as efficient tumor treatment still needs to be clarified. METHOD: In this stud...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Neoplasia Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518365/ https://www.ncbi.nlm.nih.gov/pubmed/37729741 http://dx.doi.org/10.1016/j.tranon.2023.101771 |
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author | Cai, Jiale Hu, Guiping Hu, Lihua Chen, Junge Chen, Dan Liu, Dan Wang, Xiaolei Hu, Boxian Li, Cheng |
author_facet | Cai, Jiale Hu, Guiping Hu, Lihua Chen, Junge Chen, Dan Liu, Dan Wang, Xiaolei Hu, Boxian Li, Cheng |
author_sort | Cai, Jiale |
collection | PubMed |
description | INTRODUCTION: Sonodynamic therapy (SDT) has potential clinical applications for cancer therapy, and is yet restricted by complex tumor microenvironmental (TME) factors. Thus, the research problem of TME modulation as well as efficient tumor treatment still needs to be clarified. METHOD: In this study, a calcium carbonate (CaCO(3)) nanoplatform was designed for ultrasound (US) and TME response-triggered, which encapsulated Ag(2)S and loaded with l-Arg, and further wrapped with RBC/Platelet membrane, named as QD@Ca/M(L-Arg). RESULTS: Non-invasive US-triggered SDT by Ag(2)S and acidic environment-responsive drug release were achieved. In vitro experiments validated the efficacy of SDT, Ca-ion interference and nitric oxide (NO) gas therapy as combined therapy for cancer treatment. By means of RNA sequencing, the cancer therapeutic mechanism of SDT in redox-related pathways was elucidated. The immunosuppressive TME was simulated with a M2-macrophage/cancer cell co-culture system to confirm the immune activating effect of immunogenic cell death (ICD). CONCLUSION: Accordingly, the potential of QD@Ca/M(L-)(Arg-)was demonstrated for in vitro TME modulation, cancer therapeutic efficacy and clinical translation. |
format | Online Article Text |
id | pubmed-10518365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Neoplasia Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105183652023-09-26 A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy Cai, Jiale Hu, Guiping Hu, Lihua Chen, Junge Chen, Dan Liu, Dan Wang, Xiaolei Hu, Boxian Li, Cheng Transl Oncol Original Research INTRODUCTION: Sonodynamic therapy (SDT) has potential clinical applications for cancer therapy, and is yet restricted by complex tumor microenvironmental (TME) factors. Thus, the research problem of TME modulation as well as efficient tumor treatment still needs to be clarified. METHOD: In this study, a calcium carbonate (CaCO(3)) nanoplatform was designed for ultrasound (US) and TME response-triggered, which encapsulated Ag(2)S and loaded with l-Arg, and further wrapped with RBC/Platelet membrane, named as QD@Ca/M(L-Arg). RESULTS: Non-invasive US-triggered SDT by Ag(2)S and acidic environment-responsive drug release were achieved. In vitro experiments validated the efficacy of SDT, Ca-ion interference and nitric oxide (NO) gas therapy as combined therapy for cancer treatment. By means of RNA sequencing, the cancer therapeutic mechanism of SDT in redox-related pathways was elucidated. The immunosuppressive TME was simulated with a M2-macrophage/cancer cell co-culture system to confirm the immune activating effect of immunogenic cell death (ICD). CONCLUSION: Accordingly, the potential of QD@Ca/M(L-)(Arg-)was demonstrated for in vitro TME modulation, cancer therapeutic efficacy and clinical translation. Neoplasia Press 2023-09-18 /pmc/articles/PMC10518365/ /pubmed/37729741 http://dx.doi.org/10.1016/j.tranon.2023.101771 Text en © 2023 The Authors. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Cai, Jiale Hu, Guiping Hu, Lihua Chen, Junge Chen, Dan Liu, Dan Wang, Xiaolei Hu, Boxian Li, Cheng A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy |
title | A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy |
title_full | A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy |
title_fullStr | A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy |
title_full_unstemmed | A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy |
title_short | A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy |
title_sort | caco(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518365/ https://www.ncbi.nlm.nih.gov/pubmed/37729741 http://dx.doi.org/10.1016/j.tranon.2023.101771 |
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