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A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy

INTRODUCTION: Sonodynamic therapy (SDT) has potential clinical applications for cancer therapy, and is yet restricted by complex tumor microenvironmental (TME) factors. Thus, the research problem of TME modulation as well as efficient tumor treatment still needs to be clarified. METHOD: In this stud...

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Autores principales: Cai, Jiale, Hu, Guiping, Hu, Lihua, Chen, Junge, Chen, Dan, Liu, Dan, Wang, Xiaolei, Hu, Boxian, Li, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518365/
https://www.ncbi.nlm.nih.gov/pubmed/37729741
http://dx.doi.org/10.1016/j.tranon.2023.101771
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author Cai, Jiale
Hu, Guiping
Hu, Lihua
Chen, Junge
Chen, Dan
Liu, Dan
Wang, Xiaolei
Hu, Boxian
Li, Cheng
author_facet Cai, Jiale
Hu, Guiping
Hu, Lihua
Chen, Junge
Chen, Dan
Liu, Dan
Wang, Xiaolei
Hu, Boxian
Li, Cheng
author_sort Cai, Jiale
collection PubMed
description INTRODUCTION: Sonodynamic therapy (SDT) has potential clinical applications for cancer therapy, and is yet restricted by complex tumor microenvironmental (TME) factors. Thus, the research problem of TME modulation as well as efficient tumor treatment still needs to be clarified. METHOD: In this study, a calcium carbonate (CaCO(3)) nanoplatform was designed for ultrasound (US) and TME response-triggered, which encapsulated Ag(2)S and loaded with l-Arg, and further wrapped with RBC/Platelet membrane, named as QD@Ca/M(L-Arg). RESULTS: Non-invasive US-triggered SDT by Ag(2)S and acidic environment-responsive drug release were achieved. In vitro experiments validated the efficacy of SDT, Ca-ion interference and nitric oxide (NO) gas therapy as combined therapy for cancer treatment. By means of RNA sequencing, the cancer therapeutic mechanism of SDT in redox-related pathways was elucidated. The immunosuppressive TME was simulated with a M2-macrophage/cancer cell co-culture system to confirm the immune activating effect of immunogenic cell death (ICD). CONCLUSION: Accordingly, the potential of QD@Ca/M(L-)(Arg-)was demonstrated for in vitro TME modulation, cancer therapeutic efficacy and clinical translation.
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spelling pubmed-105183652023-09-26 A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy Cai, Jiale Hu, Guiping Hu, Lihua Chen, Junge Chen, Dan Liu, Dan Wang, Xiaolei Hu, Boxian Li, Cheng Transl Oncol Original Research INTRODUCTION: Sonodynamic therapy (SDT) has potential clinical applications for cancer therapy, and is yet restricted by complex tumor microenvironmental (TME) factors. Thus, the research problem of TME modulation as well as efficient tumor treatment still needs to be clarified. METHOD: In this study, a calcium carbonate (CaCO(3)) nanoplatform was designed for ultrasound (US) and TME response-triggered, which encapsulated Ag(2)S and loaded with l-Arg, and further wrapped with RBC/Platelet membrane, named as QD@Ca/M(L-Arg). RESULTS: Non-invasive US-triggered SDT by Ag(2)S and acidic environment-responsive drug release were achieved. In vitro experiments validated the efficacy of SDT, Ca-ion interference and nitric oxide (NO) gas therapy as combined therapy for cancer treatment. By means of RNA sequencing, the cancer therapeutic mechanism of SDT in redox-related pathways was elucidated. The immunosuppressive TME was simulated with a M2-macrophage/cancer cell co-culture system to confirm the immune activating effect of immunogenic cell death (ICD). CONCLUSION: Accordingly, the potential of QD@Ca/M(L-)(Arg-)was demonstrated for in vitro TME modulation, cancer therapeutic efficacy and clinical translation. Neoplasia Press 2023-09-18 /pmc/articles/PMC10518365/ /pubmed/37729741 http://dx.doi.org/10.1016/j.tranon.2023.101771 Text en © 2023 The Authors. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research
Cai, Jiale
Hu, Guiping
Hu, Lihua
Chen, Junge
Chen, Dan
Liu, Dan
Wang, Xiaolei
Hu, Boxian
Li, Cheng
A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy
title A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy
title_full A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy
title_fullStr A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy
title_full_unstemmed A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy
title_short A CaCO(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy
title_sort caco(3)-based nanoplatform with sonodynamic and tumor microenvironment activated for combined in vitro cancer therapy
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518365/
https://www.ncbi.nlm.nih.gov/pubmed/37729741
http://dx.doi.org/10.1016/j.tranon.2023.101771
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