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Mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model

Hyperproteinemia is a metabolic disorder characterized by abnormally elevated plasma protein concentrations (PPC) in humans and animals. Here, a genetic silkworm model with high PPC was employed to investigate the effect of elevated PPC on female reproduction. Transcriptomic analysis revealed that h...

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Autores principales: Jiang, Gui-Hua, Wang, Guang, Luo, Cheng, Wang, Yong-Feng, Qiu, Jian-Feng, Peng, Ru-Ji, Sima, Yang-Hu, Xu, Shi-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518704/
https://www.ncbi.nlm.nih.gov/pubmed/37752953
http://dx.doi.org/10.1016/j.isci.2023.107860
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author Jiang, Gui-Hua
Wang, Guang
Luo, Cheng
Wang, Yong-Feng
Qiu, Jian-Feng
Peng, Ru-Ji
Sima, Yang-Hu
Xu, Shi-Qing
author_facet Jiang, Gui-Hua
Wang, Guang
Luo, Cheng
Wang, Yong-Feng
Qiu, Jian-Feng
Peng, Ru-Ji
Sima, Yang-Hu
Xu, Shi-Qing
author_sort Jiang, Gui-Hua
collection PubMed
description Hyperproteinemia is a metabolic disorder characterized by abnormally elevated plasma protein concentrations (PPC) in humans and animals. Here, a genetic silkworm model with high PPC was employed to investigate the effect of elevated PPC on female reproduction. Transcriptomic analysis revealed that high PPC induces downregulation of the ovarian development-related genes and disrupts ovarian sugar metabolism. Biochemical and endocrinal analyses revealed that high PPC increases trehalose and glucose levels in hemolymph and glycogen content in the fat body through activation of the gluconeogenic pathway and inhibition of the Insulin/Insulin-like growth factor signaling pathway-the serine/threonine kinase (IIS-AKT) pathway, thus disrupting characteristic metabolic homeostasis of sugar in the ovary. These resulted in ovarian developmental delay as well as reduced number and poor quality of eggs. Insulin supplementation effectively increased egg numbers by lowering blood sugar. These collective results provide new insights into the mechanisms by which high PPC negatively affects female reproduction and support the potential therapeutic effects of insulin.
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spelling pubmed-105187042023-09-26 Mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model Jiang, Gui-Hua Wang, Guang Luo, Cheng Wang, Yong-Feng Qiu, Jian-Feng Peng, Ru-Ji Sima, Yang-Hu Xu, Shi-Qing iScience Article Hyperproteinemia is a metabolic disorder characterized by abnormally elevated plasma protein concentrations (PPC) in humans and animals. Here, a genetic silkworm model with high PPC was employed to investigate the effect of elevated PPC on female reproduction. Transcriptomic analysis revealed that high PPC induces downregulation of the ovarian development-related genes and disrupts ovarian sugar metabolism. Biochemical and endocrinal analyses revealed that high PPC increases trehalose and glucose levels in hemolymph and glycogen content in the fat body through activation of the gluconeogenic pathway and inhibition of the Insulin/Insulin-like growth factor signaling pathway-the serine/threonine kinase (IIS-AKT) pathway, thus disrupting characteristic metabolic homeostasis of sugar in the ovary. These resulted in ovarian developmental delay as well as reduced number and poor quality of eggs. Insulin supplementation effectively increased egg numbers by lowering blood sugar. These collective results provide new insights into the mechanisms by which high PPC negatively affects female reproduction and support the potential therapeutic effects of insulin. Elsevier 2023-09-09 /pmc/articles/PMC10518704/ /pubmed/37752953 http://dx.doi.org/10.1016/j.isci.2023.107860 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Jiang, Gui-Hua
Wang, Guang
Luo, Cheng
Wang, Yong-Feng
Qiu, Jian-Feng
Peng, Ru-Ji
Sima, Yang-Hu
Xu, Shi-Qing
Mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model
title Mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model
title_full Mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model
title_fullStr Mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model
title_full_unstemmed Mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model
title_short Mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model
title_sort mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518704/
https://www.ncbi.nlm.nih.gov/pubmed/37752953
http://dx.doi.org/10.1016/j.isci.2023.107860
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