Cargando…
Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice
BACKGROUND: Di (2-ethylhexyl) phthalate (DEHP) is a common plasticizer known to cause liver injury. Green tea is reported to exert therapeutic effects on heavy metal exposure-induced organ damage. However, limited studies have examined the therapeutic effects of green tea polyphenols (GTPs) on DEHP-...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518738/ https://www.ncbi.nlm.nih.gov/pubmed/37753369 http://dx.doi.org/10.3748/wjg.v29.i34.5054 |
_version_ | 1785109581645479936 |
---|---|
author | Shi, Heng Zhao, Xin-Hai Peng, Qin Zhou, Xian-Ling Liu, Si-Si Sun, Chuan-Chuan Cao, Qiu-Yu Zhu, Shi-Ping Sun, Sheng-Yun |
author_facet | Shi, Heng Zhao, Xin-Hai Peng, Qin Zhou, Xian-Ling Liu, Si-Si Sun, Chuan-Chuan Cao, Qiu-Yu Zhu, Shi-Ping Sun, Sheng-Yun |
author_sort | Shi, Heng |
collection | PubMed |
description | BACKGROUND: Di (2-ethylhexyl) phthalate (DEHP) is a common plasticizer known to cause liver injury. Green tea is reported to exert therapeutic effects on heavy metal exposure-induced organ damage. However, limited studies have examined the therapeutic effects of green tea polyphenols (GTPs) on DEHP-induced liver damage. AIM: To evaluate the molecular mechanism underlying the therapeutic effects of GTPs on DEHP-induced liver damage. METHODS: C57BL/6J mice were divided into the following five groups: Control, model [DEHP (1500 mg/kg bodyweight)], treatment [DEHP (1500 mg/kg bodyweight) + GTP (70 mg/kg bodyweight), oil, and GTP (70 mg/kg bodyweight)] groups. After 8 wk, the liver function, blood lipid profile, and liver histopathology were examined. Differentially expressed micro RNAs (miRNAs) and mRNAs in the liver tissues were examined using high-throughput sequencing. Additionally, functional enrichment analysis and immune infiltration prediction were performed. The miRNA-mRNA regulatory axis was elucidated using the starBase database. Protein expression was evaluated using immunohistochemistry. RESULTS: GTPs alleviated DHEP-induced liver dysfunction, blood lipid dysregulation, fatty liver disease, liver fibrosis, and mitochondrial and endoplasmic reticulum lesions in mice. The infiltration of macrophages, mast cells, and natural killer cells varied between the model and treatment groups. mmu-miR-141-3p (a differentially expressed miRNA), Zcchc24 (a differentially expressed mRNA), and Zcchc24 (a differentially expressed protein) constituted the miRNA-mRNA-protein regulatory axis involved in mediating the therapeutic effects of GTPs on DEHP-induced liver damage in mice. CONCLUSION: This study demonstrated that GTPs mitigate DEHP-induced liver dysfunction, blood lipid dysregulation, fatty liver disease, and partial liver fibrosis, and regulate immune cell infiltration. Additionally, an important miRNA-mRNA-protein molecular regulatory axis involved in mediating the therapeutic effects of GTPs on DEHP-induced liver damage was elucidated. |
format | Online Article Text |
id | pubmed-10518738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-105187382023-09-26 Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice Shi, Heng Zhao, Xin-Hai Peng, Qin Zhou, Xian-Ling Liu, Si-Si Sun, Chuan-Chuan Cao, Qiu-Yu Zhu, Shi-Ping Sun, Sheng-Yun World J Gastroenterol Basic Study BACKGROUND: Di (2-ethylhexyl) phthalate (DEHP) is a common plasticizer known to cause liver injury. Green tea is reported to exert therapeutic effects on heavy metal exposure-induced organ damage. However, limited studies have examined the therapeutic effects of green tea polyphenols (GTPs) on DEHP-induced liver damage. AIM: To evaluate the molecular mechanism underlying the therapeutic effects of GTPs on DEHP-induced liver damage. METHODS: C57BL/6J mice were divided into the following five groups: Control, model [DEHP (1500 mg/kg bodyweight)], treatment [DEHP (1500 mg/kg bodyweight) + GTP (70 mg/kg bodyweight), oil, and GTP (70 mg/kg bodyweight)] groups. After 8 wk, the liver function, blood lipid profile, and liver histopathology were examined. Differentially expressed micro RNAs (miRNAs) and mRNAs in the liver tissues were examined using high-throughput sequencing. Additionally, functional enrichment analysis and immune infiltration prediction were performed. The miRNA-mRNA regulatory axis was elucidated using the starBase database. Protein expression was evaluated using immunohistochemistry. RESULTS: GTPs alleviated DHEP-induced liver dysfunction, blood lipid dysregulation, fatty liver disease, liver fibrosis, and mitochondrial and endoplasmic reticulum lesions in mice. The infiltration of macrophages, mast cells, and natural killer cells varied between the model and treatment groups. mmu-miR-141-3p (a differentially expressed miRNA), Zcchc24 (a differentially expressed mRNA), and Zcchc24 (a differentially expressed protein) constituted the miRNA-mRNA-protein regulatory axis involved in mediating the therapeutic effects of GTPs on DEHP-induced liver damage in mice. CONCLUSION: This study demonstrated that GTPs mitigate DEHP-induced liver dysfunction, blood lipid dysregulation, fatty liver disease, and partial liver fibrosis, and regulate immune cell infiltration. Additionally, an important miRNA-mRNA-protein molecular regulatory axis involved in mediating the therapeutic effects of GTPs on DEHP-induced liver damage was elucidated. Baishideng Publishing Group Inc 2023-09-14 2023-09-14 /pmc/articles/PMC10518738/ /pubmed/37753369 http://dx.doi.org/10.3748/wjg.v29.i34.5054 Text en ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
spellingShingle | Basic Study Shi, Heng Zhao, Xin-Hai Peng, Qin Zhou, Xian-Ling Liu, Si-Si Sun, Chuan-Chuan Cao, Qiu-Yu Zhu, Shi-Ping Sun, Sheng-Yun Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice |
title | Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice |
title_full | Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice |
title_fullStr | Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice |
title_full_unstemmed | Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice |
title_short | Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice |
title_sort | green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518738/ https://www.ncbi.nlm.nih.gov/pubmed/37753369 http://dx.doi.org/10.3748/wjg.v29.i34.5054 |
work_keys_str_mv | AT shiheng greenteapolyphenolsalleviatedi2ethylhexylphthalateinducedliverinjuryinmice AT zhaoxinhai greenteapolyphenolsalleviatedi2ethylhexylphthalateinducedliverinjuryinmice AT pengqin greenteapolyphenolsalleviatedi2ethylhexylphthalateinducedliverinjuryinmice AT zhouxianling greenteapolyphenolsalleviatedi2ethylhexylphthalateinducedliverinjuryinmice AT liusisi greenteapolyphenolsalleviatedi2ethylhexylphthalateinducedliverinjuryinmice AT sunchuanchuan greenteapolyphenolsalleviatedi2ethylhexylphthalateinducedliverinjuryinmice AT caoqiuyu greenteapolyphenolsalleviatedi2ethylhexylphthalateinducedliverinjuryinmice AT zhushiping greenteapolyphenolsalleviatedi2ethylhexylphthalateinducedliverinjuryinmice AT sunshengyun greenteapolyphenolsalleviatedi2ethylhexylphthalateinducedliverinjuryinmice |