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Urine proteomic analysis of the rat e-cigarette model
BACKGROUND: We were curious if the urinary proteome could reflect the effects of e-cigarettes on the organism. METHODS: Urine samples were collected from a rat e-cigarette model before, during, and after two weeks of e-cigarette smoking. Urine proteomes before and after smoking of each rat were comp...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519197/ https://www.ncbi.nlm.nih.gov/pubmed/37753171 http://dx.doi.org/10.7717/peerj.16041 |
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author | Liu, Yuqing Shen, Ziyun Zhao, Chenyang Gao, Youhe |
author_facet | Liu, Yuqing Shen, Ziyun Zhao, Chenyang Gao, Youhe |
author_sort | Liu, Yuqing |
collection | PubMed |
description | BACKGROUND: We were curious if the urinary proteome could reflect the effects of e-cigarettes on the organism. METHODS: Urine samples were collected from a rat e-cigarette model before, during, and after two weeks of e-cigarette smoking. Urine proteomes before and after smoking of each rat were compared individually, while the control group was set up to rule out differences caused by rat growth and development. RESULTS: Fetuin-B, a biomarker of chronic obstructive pulmonary disease (COPD), and annexin A2, which is recognized as a multiple tumour marker, were identified as differential proteins in five out of six smoking rats on day 3. To our surprise, odourant-binding proteins expressed in the olfactory epithelium were also found and were significantly upregulated. Pathways enriched by the differential proteins include the apelin signalling pathway, folate biosynthesis pathway, arachidonic acid metabolism, chemical carcinogenesis-DNA adducts and chemical carcinogenesis-reactive oxygen species. They have been reported to be associated with immune system, cardiovascular system, respiratory system, etc. CONCLUSIONS: Urinary proteome could reflect the effects of e-cigarettes in rats. |
format | Online Article Text |
id | pubmed-10519197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105191972023-09-26 Urine proteomic analysis of the rat e-cigarette model Liu, Yuqing Shen, Ziyun Zhao, Chenyang Gao, Youhe PeerJ Biochemistry BACKGROUND: We were curious if the urinary proteome could reflect the effects of e-cigarettes on the organism. METHODS: Urine samples were collected from a rat e-cigarette model before, during, and after two weeks of e-cigarette smoking. Urine proteomes before and after smoking of each rat were compared individually, while the control group was set up to rule out differences caused by rat growth and development. RESULTS: Fetuin-B, a biomarker of chronic obstructive pulmonary disease (COPD), and annexin A2, which is recognized as a multiple tumour marker, were identified as differential proteins in five out of six smoking rats on day 3. To our surprise, odourant-binding proteins expressed in the olfactory epithelium were also found and were significantly upregulated. Pathways enriched by the differential proteins include the apelin signalling pathway, folate biosynthesis pathway, arachidonic acid metabolism, chemical carcinogenesis-DNA adducts and chemical carcinogenesis-reactive oxygen species. They have been reported to be associated with immune system, cardiovascular system, respiratory system, etc. CONCLUSIONS: Urinary proteome could reflect the effects of e-cigarettes in rats. PeerJ Inc. 2023-09-22 /pmc/articles/PMC10519197/ /pubmed/37753171 http://dx.doi.org/10.7717/peerj.16041 Text en ©2023 Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biochemistry Liu, Yuqing Shen, Ziyun Zhao, Chenyang Gao, Youhe Urine proteomic analysis of the rat e-cigarette model |
title | Urine proteomic analysis of the rat e-cigarette model |
title_full | Urine proteomic analysis of the rat e-cigarette model |
title_fullStr | Urine proteomic analysis of the rat e-cigarette model |
title_full_unstemmed | Urine proteomic analysis of the rat e-cigarette model |
title_short | Urine proteomic analysis of the rat e-cigarette model |
title_sort | urine proteomic analysis of the rat e-cigarette model |
topic | Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519197/ https://www.ncbi.nlm.nih.gov/pubmed/37753171 http://dx.doi.org/10.7717/peerj.16041 |
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