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Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants

While bacterial diversity is beneficial for the functioning of rhizosphere microbiomes, multi-species bioinoculants often fail to promote plant growth. One potential reason for this is that competition between different species of inoculated consortia members creates conflicts for their survival and...

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Autores principales: Li, Jingxuan, Yang, Chunlan, Jousset, Alexandre, Yang, Keming, Wang, Xiaofang, Xu, Zhihui, Yang, Tianjie, Mei, Xinlan, Zhong, Zengtao, Xu, Yangchun, Shen, Qirong, Friman, Ville-Petri, Wei, Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519709/
https://www.ncbi.nlm.nih.gov/pubmed/37706503
http://dx.doi.org/10.7554/eLife.90726
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author Li, Jingxuan
Yang, Chunlan
Jousset, Alexandre
Yang, Keming
Wang, Xiaofang
Xu, Zhihui
Yang, Tianjie
Mei, Xinlan
Zhong, Zengtao
Xu, Yangchun
Shen, Qirong
Friman, Ville-Petri
Wei, Zhong
author_facet Li, Jingxuan
Yang, Chunlan
Jousset, Alexandre
Yang, Keming
Wang, Xiaofang
Xu, Zhihui
Yang, Tianjie
Mei, Xinlan
Zhong, Zengtao
Xu, Yangchun
Shen, Qirong
Friman, Ville-Petri
Wei, Zhong
author_sort Li, Jingxuan
collection PubMed
description While bacterial diversity is beneficial for the functioning of rhizosphere microbiomes, multi-species bioinoculants often fail to promote plant growth. One potential reason for this is that competition between different species of inoculated consortia members creates conflicts for their survival and functioning. To circumvent this, we used transposon insertion mutagenesis to increase the functional diversity within Bacillus amyloliquefaciens bacterial species and tested if we could improve plant growth promotion by assembling consortia of highly clonal but phenotypically dissimilar mutants. While most insertion mutations were harmful, some significantly improved B. amyloliquefaciens plant growth promotion traits relative to the wild-type strain. Eight phenotypically distinct mutants were selected to test if their functioning could be improved by applying them as multifunctional consortia. We found that B. amyloliquefaciens consortium richness correlated positively with plant root colonization and protection from Ralstonia solanacearum phytopathogenic bacterium. Crucially, 8-mutant consortium consisting of phenotypically dissimilar mutants performed better than randomly assembled 8-mutant consortia, suggesting that improvements were likely driven by consortia multifunctionality instead of consortia richness. Together, our results suggest that increasing intra-species phenotypic diversity could be an effective way to improve probiotic consortium functioning and plant growth promotion in agricultural systems.
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spelling pubmed-105197092023-09-26 Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants Li, Jingxuan Yang, Chunlan Jousset, Alexandre Yang, Keming Wang, Xiaofang Xu, Zhihui Yang, Tianjie Mei, Xinlan Zhong, Zengtao Xu, Yangchun Shen, Qirong Friman, Ville-Petri Wei, Zhong eLife Ecology While bacterial diversity is beneficial for the functioning of rhizosphere microbiomes, multi-species bioinoculants often fail to promote plant growth. One potential reason for this is that competition between different species of inoculated consortia members creates conflicts for their survival and functioning. To circumvent this, we used transposon insertion mutagenesis to increase the functional diversity within Bacillus amyloliquefaciens bacterial species and tested if we could improve plant growth promotion by assembling consortia of highly clonal but phenotypically dissimilar mutants. While most insertion mutations were harmful, some significantly improved B. amyloliquefaciens plant growth promotion traits relative to the wild-type strain. Eight phenotypically distinct mutants were selected to test if their functioning could be improved by applying them as multifunctional consortia. We found that B. amyloliquefaciens consortium richness correlated positively with plant root colonization and protection from Ralstonia solanacearum phytopathogenic bacterium. Crucially, 8-mutant consortium consisting of phenotypically dissimilar mutants performed better than randomly assembled 8-mutant consortia, suggesting that improvements were likely driven by consortia multifunctionality instead of consortia richness. Together, our results suggest that increasing intra-species phenotypic diversity could be an effective way to improve probiotic consortium functioning and plant growth promotion in agricultural systems. eLife Sciences Publications, Ltd 2023-09-14 /pmc/articles/PMC10519709/ /pubmed/37706503 http://dx.doi.org/10.7554/eLife.90726 Text en © 2023, Li, Yang et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Ecology
Li, Jingxuan
Yang, Chunlan
Jousset, Alexandre
Yang, Keming
Wang, Xiaofang
Xu, Zhihui
Yang, Tianjie
Mei, Xinlan
Zhong, Zengtao
Xu, Yangchun
Shen, Qirong
Friman, Ville-Petri
Wei, Zhong
Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants
title Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants
title_full Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants
title_fullStr Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants
title_full_unstemmed Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants
title_short Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants
title_sort engineering multifunctional rhizosphere probiotics using consortia of bacillus amyloliquefaciens transposon insertion mutants
topic Ecology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519709/
https://www.ncbi.nlm.nih.gov/pubmed/37706503
http://dx.doi.org/10.7554/eLife.90726
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