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The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation
Mesenchymal stem cells (MSCs) play a pivotal role in tissue engineering and regenerative medicine, with their clinical application often hindered by cell senescence during ex vivo expansion. Recent studies suggest that MSC-deposited decellularized extracellular matrix (dECM) offers a conducive micro...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519827/ https://www.ncbi.nlm.nih.gov/pubmed/37766896 http://dx.doi.org/10.1016/j.mtbio.2023.100805 |
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author | Pei, Yixuan Amy Mikaeiliagah, Elmira Wang, Bin Zhang, Xiaobing Pei, Ming |
author_facet | Pei, Yixuan Amy Mikaeiliagah, Elmira Wang, Bin Zhang, Xiaobing Pei, Ming |
author_sort | Pei, Yixuan Amy |
collection | PubMed |
description | Mesenchymal stem cells (MSCs) play a pivotal role in tissue engineering and regenerative medicine, with their clinical application often hindered by cell senescence during ex vivo expansion. Recent studies suggest that MSC-deposited decellularized extracellular matrix (dECM) offers a conducive microenvironment that fosters cell proliferation and accentuates stem cell differentiation. However, the ability of this matrix environment to govern lineage differentiation of tissue-specific stem cells remains ambiguous. This research employs human adipose-derived MSCs (ADSCs) and synovium-derived MSCs (SDSCs) as models for adipogenesis and chondrogenesis differentiation pathways, respectively. Genetically modified dECM (GMdECM), produced by SV40LT-transduced immortalized cells, was studied for its influence on cell differentiation. Both types of immortalized cells displayed a reduction in chondrogenic ability but an enhancement in adipogenic potential. ADSCs grown on ADSC-deposited dECM showed stable chondrogenic potential but increased adipogenic capacity; conversely, SDSCs expanded on SDSC-generated dECM displayed elevated chondrogenic capacity and diminished adipogenic potential. This cell-dependent response was confirmed through GMdECM expansion, with SDSCs showing enhanced chondrogenesis. However, ADSCs did not exhibit improved chondrogenic potential on GMdECM, suggesting that the matrix microenvironment does not dictate the final differentiation path of tissue-specific stem cells. Potential molecular mechanisms, such as elevated basement membrane protein expression in GMdECMs and dynamic TWIST1 expression during expansion and chondrogenic induction, may underpin the strong chondrogenic differentiation of GMdECM-expanded SDSCs. |
format | Online Article Text |
id | pubmed-10519827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105198272023-09-27 The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation Pei, Yixuan Amy Mikaeiliagah, Elmira Wang, Bin Zhang, Xiaobing Pei, Ming Mater Today Bio Full Length Article Mesenchymal stem cells (MSCs) play a pivotal role in tissue engineering and regenerative medicine, with their clinical application often hindered by cell senescence during ex vivo expansion. Recent studies suggest that MSC-deposited decellularized extracellular matrix (dECM) offers a conducive microenvironment that fosters cell proliferation and accentuates stem cell differentiation. However, the ability of this matrix environment to govern lineage differentiation of tissue-specific stem cells remains ambiguous. This research employs human adipose-derived MSCs (ADSCs) and synovium-derived MSCs (SDSCs) as models for adipogenesis and chondrogenesis differentiation pathways, respectively. Genetically modified dECM (GMdECM), produced by SV40LT-transduced immortalized cells, was studied for its influence on cell differentiation. Both types of immortalized cells displayed a reduction in chondrogenic ability but an enhancement in adipogenic potential. ADSCs grown on ADSC-deposited dECM showed stable chondrogenic potential but increased adipogenic capacity; conversely, SDSCs expanded on SDSC-generated dECM displayed elevated chondrogenic capacity and diminished adipogenic potential. This cell-dependent response was confirmed through GMdECM expansion, with SDSCs showing enhanced chondrogenesis. However, ADSCs did not exhibit improved chondrogenic potential on GMdECM, suggesting that the matrix microenvironment does not dictate the final differentiation path of tissue-specific stem cells. Potential molecular mechanisms, such as elevated basement membrane protein expression in GMdECMs and dynamic TWIST1 expression during expansion and chondrogenic induction, may underpin the strong chondrogenic differentiation of GMdECM-expanded SDSCs. Elsevier 2023-09-16 /pmc/articles/PMC10519827/ /pubmed/37766896 http://dx.doi.org/10.1016/j.mtbio.2023.100805 Text en © 2023 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Full Length Article Pei, Yixuan Amy Mikaeiliagah, Elmira Wang, Bin Zhang, Xiaobing Pei, Ming The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation |
title | The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation |
title_full | The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation |
title_fullStr | The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation |
title_full_unstemmed | The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation |
title_short | The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation |
title_sort | matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519827/ https://www.ncbi.nlm.nih.gov/pubmed/37766896 http://dx.doi.org/10.1016/j.mtbio.2023.100805 |
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