Cargando…
Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans
The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519838/ https://www.ncbi.nlm.nih.gov/pubmed/37733596 http://dx.doi.org/10.1016/j.xpro.2023.102576 |
_version_ | 1785109781821784064 |
---|---|
author | Scholte, Larissa L.S. Leggat, David J. Cohen, Kristen W. Hoeweler, Lara Erwin, Guacyara C. Rahaman, Farhard Lombardo, Angela Philiponis, Vincent Laufer, Dagna S. Siefers, Heather Ruppel, Alexis M. Brand, Joshua Maenza, Janine Bronson, Rhi Prabhakaran, Madhu Jean-Baptiste, Jalen Kolokythas, Orpheus Desrosiers, Aimee A. Thoreson, Caroline K. Heit, Antje Khati, Nadia J. Malkin, Elissa McElrath, M. Juliana McDermott, Adrian B. Schief, William R. Diemert, David Bethony, Jeffrey M. |
author_facet | Scholte, Larissa L.S. Leggat, David J. Cohen, Kristen W. Hoeweler, Lara Erwin, Guacyara C. Rahaman, Farhard Lombardo, Angela Philiponis, Vincent Laufer, Dagna S. Siefers, Heather Ruppel, Alexis M. Brand, Joshua Maenza, Janine Bronson, Rhi Prabhakaran, Madhu Jean-Baptiste, Jalen Kolokythas, Orpheus Desrosiers, Aimee A. Thoreson, Caroline K. Heit, Antje Khati, Nadia J. Malkin, Elissa McElrath, M. Juliana McDermott, Adrian B. Schief, William R. Diemert, David Bethony, Jeffrey M. |
author_sort | Scholte, Larissa L.S. |
collection | PubMed |
description | The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022).(1) |
format | Online Article Text |
id | pubmed-10519838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105198382023-09-27 Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans Scholte, Larissa L.S. Leggat, David J. Cohen, Kristen W. Hoeweler, Lara Erwin, Guacyara C. Rahaman, Farhard Lombardo, Angela Philiponis, Vincent Laufer, Dagna S. Siefers, Heather Ruppel, Alexis M. Brand, Joshua Maenza, Janine Bronson, Rhi Prabhakaran, Madhu Jean-Baptiste, Jalen Kolokythas, Orpheus Desrosiers, Aimee A. Thoreson, Caroline K. Heit, Antje Khati, Nadia J. Malkin, Elissa McElrath, M. Juliana McDermott, Adrian B. Schief, William R. Diemert, David Bethony, Jeffrey M. STAR Protoc Protocol The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022).(1) Elsevier 2023-09-20 /pmc/articles/PMC10519838/ /pubmed/37733596 http://dx.doi.org/10.1016/j.xpro.2023.102576 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Scholte, Larissa L.S. Leggat, David J. Cohen, Kristen W. Hoeweler, Lara Erwin, Guacyara C. Rahaman, Farhard Lombardo, Angela Philiponis, Vincent Laufer, Dagna S. Siefers, Heather Ruppel, Alexis M. Brand, Joshua Maenza, Janine Bronson, Rhi Prabhakaran, Madhu Jean-Baptiste, Jalen Kolokythas, Orpheus Desrosiers, Aimee A. Thoreson, Caroline K. Heit, Antje Khati, Nadia J. Malkin, Elissa McElrath, M. Juliana McDermott, Adrian B. Schief, William R. Diemert, David Bethony, Jeffrey M. Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans |
title | Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans |
title_full | Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans |
title_fullStr | Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans |
title_full_unstemmed | Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans |
title_short | Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans |
title_sort | ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519838/ https://www.ncbi.nlm.nih.gov/pubmed/37733596 http://dx.doi.org/10.1016/j.xpro.2023.102576 |
work_keys_str_mv | AT scholtelarissals ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT leggatdavidj ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT cohenkristenw ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT hoewelerlara ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT erwinguacyarac ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT rahamanfarhard ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT lombardoangela ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT philiponisvincent ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT lauferdagnas ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT siefersheather ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT ruppelalexism ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT brandjoshua ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT maenzajanine ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT bronsonrhi ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT prabhakaranmadhu ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT jeanbaptistejalen ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT kolokythasorpheus ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT desrosiersaimeea ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT thoresoncarolinek ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT heitantje ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT khatinadiaj ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT malkinelissa ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT mcelrathmjuliana ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT mcdermottadrianb ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT schiefwilliamr ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT diemertdavid ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans AT bethonyjeffreym ultrasoundguidedlymphnodefineneedleaspirationforevaluatingpostvaccinationgerminalcenterresponsesinhumans |