Protocol for reconstitution of oligomeric assembly of NAIP5-NLRC4 inflammasome in vitro

Inflammasomes are multimeric protein complexes that have crucial functions in innate immunity. Here, we present a protocol to reconstitute the PELO-driven assembly of NAIP5-NLRC4 inflammasome in vitro. We describe steps for expression and purification of recombinant PELO and flagellin, preparation o...

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Detalles Bibliográficos
Autores principales: Wu, Xiurong, Han, Jiahuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519842/
https://www.ncbi.nlm.nih.gov/pubmed/37733592
http://dx.doi.org/10.1016/j.xpro.2023.102581

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