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Protocol for high-sensitivity western blot on murine hematopoietic stem cells

Hematopoietic stem cells (HSCs) sustain hematopoiesis during homeostasis and regeneration. However, their limited availability poses a challenge for protein analysis. Here, we present a protocol for performing high-sensitivity western blot on HSCs using two techniques that enhance HSC isolation from...

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Detalles Bibliográficos
Autores principales: Li, Changzheng, Guan, Jingjing, Li, Yishan, Tian, Xiaobin, Zhao, Yijun, Liu, Weiming, Tian, Huixuan, Tian, Huiqi, Yang, Yalan, Zhao, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519847/
https://www.ncbi.nlm.nih.gov/pubmed/37733599
http://dx.doi.org/10.1016/j.xpro.2023.102578

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