Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors

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Here, we present a protocol for producing 3D pancreatic-like organoids from human pluripotent stem cells in suspension bioreactors. We describe scalable techniques for generating 10,000–100,000 organoids that further mature in 4–5 weeks into α- and β-like cells with glucose-responsive insulin and gl...

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Detalles Bibliográficos
Autores principales: Pollock, Samuel D., Galicia-Silva, Israeli M., Liu, Mai, Gruskin, Zoe L., Alvarez-Dominguez, Juan R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519857/
https://www.ncbi.nlm.nih.gov/pubmed/37738117
http://dx.doi.org/10.1016/j.xpro.2023.102580

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