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Genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (Sesamum indicum L.) under abiotic stress

BACKGROUND: The plant trihelix gene family is among the earliest discovered transcription factor families, and it is vital in modulating light, plant growth, and stress responses. METHODS: The identification and characterization of trihelix family members in the sesame genome were analyzed by bioinf...

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Autores principales: Zhao, Yunyan, Liang, Junchao, Wang, Zhiqi, Yan, Tingxian, Yan, Xiaowen, Wei, Wenliang, Le, Meiwang, Sun, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519867/
https://www.ncbi.nlm.nih.gov/pubmed/37584845
http://dx.doi.org/10.1007/s11033-023-08640-w
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author Zhao, Yunyan
Liang, Junchao
Wang, Zhiqi
Yan, Tingxian
Yan, Xiaowen
Wei, Wenliang
Le, Meiwang
Sun, Jian
author_facet Zhao, Yunyan
Liang, Junchao
Wang, Zhiqi
Yan, Tingxian
Yan, Xiaowen
Wei, Wenliang
Le, Meiwang
Sun, Jian
author_sort Zhao, Yunyan
collection PubMed
description BACKGROUND: The plant trihelix gene family is among the earliest discovered transcription factor families, and it is vital in modulating light, plant growth, and stress responses. METHODS: The identification and characterization of trihelix family members in the sesame genome were analyzed by bioinformatics methods, and the expression patterns of sesame trihelix genes were assessed by quantitative real-time PCR. RESULTS: There were 34 trihelix genes discovered in the genome of sesame, which were irregularly distributed among 10 linkage groups. Also, the genome contained 5 duplicate gene pairs. The 34 trihelix genes were divided into six sub-families through a phylogenetic study. A tissue-specific expression revealed that SiTH genes exhibited spatial expression patterns distinct from other trihelix genes in the same subfamily. The cis-element showed that the SiTHs gene promoter contained various elements associated with responses to hormones and multiple abiotic stresses. Additionally, the expression patterns of 8 SiTH genes in leaves under abiotic stresses demonstrated that all selected genes were significantly upregulated or downregulated at least once in the stress period. Furthermore, the SiTH4 gene was significantly induced in response to drought and salt stress, showing that SiTH genes may be engaged in the stress response mechanisms of sesame. CONCLUSION: These findings establish a foundation for further investigation of the trihelix gene-mediated response to abiotic stress in sesame. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11033-023-08640-w.
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spelling pubmed-105198672023-09-27 Genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (Sesamum indicum L.) under abiotic stress Zhao, Yunyan Liang, Junchao Wang, Zhiqi Yan, Tingxian Yan, Xiaowen Wei, Wenliang Le, Meiwang Sun, Jian Mol Biol Rep Original Article BACKGROUND: The plant trihelix gene family is among the earliest discovered transcription factor families, and it is vital in modulating light, plant growth, and stress responses. METHODS: The identification and characterization of trihelix family members in the sesame genome were analyzed by bioinformatics methods, and the expression patterns of sesame trihelix genes were assessed by quantitative real-time PCR. RESULTS: There were 34 trihelix genes discovered in the genome of sesame, which were irregularly distributed among 10 linkage groups. Also, the genome contained 5 duplicate gene pairs. The 34 trihelix genes were divided into six sub-families through a phylogenetic study. A tissue-specific expression revealed that SiTH genes exhibited spatial expression patterns distinct from other trihelix genes in the same subfamily. The cis-element showed that the SiTHs gene promoter contained various elements associated with responses to hormones and multiple abiotic stresses. Additionally, the expression patterns of 8 SiTH genes in leaves under abiotic stresses demonstrated that all selected genes were significantly upregulated or downregulated at least once in the stress period. Furthermore, the SiTH4 gene was significantly induced in response to drought and salt stress, showing that SiTH genes may be engaged in the stress response mechanisms of sesame. CONCLUSION: These findings establish a foundation for further investigation of the trihelix gene-mediated response to abiotic stress in sesame. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11033-023-08640-w. Springer Netherlands 2023-08-16 2023 /pmc/articles/PMC10519867/ /pubmed/37584845 http://dx.doi.org/10.1007/s11033-023-08640-w Text en © The Author(s) 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Zhao, Yunyan
Liang, Junchao
Wang, Zhiqi
Yan, Tingxian
Yan, Xiaowen
Wei, Wenliang
Le, Meiwang
Sun, Jian
Genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (Sesamum indicum L.) under abiotic stress
title Genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (Sesamum indicum L.) under abiotic stress
title_full Genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (Sesamum indicum L.) under abiotic stress
title_fullStr Genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (Sesamum indicum L.) under abiotic stress
title_full_unstemmed Genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (Sesamum indicum L.) under abiotic stress
title_short Genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (Sesamum indicum L.) under abiotic stress
title_sort genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (sesamum indicum l.) under abiotic stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519867/
https://www.ncbi.nlm.nih.gov/pubmed/37584845
http://dx.doi.org/10.1007/s11033-023-08640-w
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