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Thromboxane prostanoid signaling in macrophages attenuates lymphedema and facilitates lymphangiogenesis in mice: TP signaling and lymphangiogenesis
BACKGROUND: Accumulating evidence suggests that prostaglandin E(2), an arachidonic acid (AA) metabolite, enhances lymphangiogenesis in response to inflammation. However, thromboxane A(2) (TXA(2)), another AA metabolite, is not well known. Thus, this study aimed to determine the role of thromboxane p...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520203/ https://www.ncbi.nlm.nih.gov/pubmed/37540456 http://dx.doi.org/10.1007/s11033-023-08620-0 |
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author | Mishima, Toshiaki Hosono, Kanako Tanabe, Mina Ito, Yoshiya Majima, Masataka Narumiya, Shuh Miyaji, Kagami Amano, Hideki |
author_facet | Mishima, Toshiaki Hosono, Kanako Tanabe, Mina Ito, Yoshiya Majima, Masataka Narumiya, Shuh Miyaji, Kagami Amano, Hideki |
author_sort | Mishima, Toshiaki |
collection | PubMed |
description | BACKGROUND: Accumulating evidence suggests that prostaglandin E(2), an arachidonic acid (AA) metabolite, enhances lymphangiogenesis in response to inflammation. However, thromboxane A(2) (TXA(2)), another AA metabolite, is not well known. Thus, this study aimed to determine the role of thromboxane prostanoid (TP) signaling in lymphangiogenesis in secondary lymphedema. METHODS AND RESULTS: Lymphedema was induced by the ablation of lymphatic vessels in mouse tails. Compared with wild-type mice, tail lymphedema in Tp-deficient mice was enhanced, which was associated with suppressed lymphangiogenesis as indicated by decreased lymphatic vessel area and pro-lymphangiogenesis-stimulating factors. Numerous macrophages were found in the tail tissues of Tp-deficient mice. Furthermore, the deletion of TP in macrophages increased tail edema and decreased lymphangiogenesis and pro-lymphangiogenic cytokines, which was accompanied by increased numbers of macrophages and gene expression related to a pro-inflammatory macrophage phenotype in tail tissues. In vivo microscopic studies revealed fluorescent dye leakage in the lymphatic vessels in the wounded tissues. CONCLUSIONS: The results suggest that TP signaling in macrophages promotes lymphangiogenesis and prevents tail lymphedema. TP signaling may be a therapeutic target for improving lymphedema-related symptoms by enhancing lymphangiogenesis. |
format | Online Article Text |
id | pubmed-10520203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-105202032023-09-27 Thromboxane prostanoid signaling in macrophages attenuates lymphedema and facilitates lymphangiogenesis in mice: TP signaling and lymphangiogenesis Mishima, Toshiaki Hosono, Kanako Tanabe, Mina Ito, Yoshiya Majima, Masataka Narumiya, Shuh Miyaji, Kagami Amano, Hideki Mol Biol Rep Original Article BACKGROUND: Accumulating evidence suggests that prostaglandin E(2), an arachidonic acid (AA) metabolite, enhances lymphangiogenesis in response to inflammation. However, thromboxane A(2) (TXA(2)), another AA metabolite, is not well known. Thus, this study aimed to determine the role of thromboxane prostanoid (TP) signaling in lymphangiogenesis in secondary lymphedema. METHODS AND RESULTS: Lymphedema was induced by the ablation of lymphatic vessels in mouse tails. Compared with wild-type mice, tail lymphedema in Tp-deficient mice was enhanced, which was associated with suppressed lymphangiogenesis as indicated by decreased lymphatic vessel area and pro-lymphangiogenesis-stimulating factors. Numerous macrophages were found in the tail tissues of Tp-deficient mice. Furthermore, the deletion of TP in macrophages increased tail edema and decreased lymphangiogenesis and pro-lymphangiogenic cytokines, which was accompanied by increased numbers of macrophages and gene expression related to a pro-inflammatory macrophage phenotype in tail tissues. In vivo microscopic studies revealed fluorescent dye leakage in the lymphatic vessels in the wounded tissues. CONCLUSIONS: The results suggest that TP signaling in macrophages promotes lymphangiogenesis and prevents tail lymphedema. TP signaling may be a therapeutic target for improving lymphedema-related symptoms by enhancing lymphangiogenesis. Springer Netherlands 2023-08-04 2023 /pmc/articles/PMC10520203/ /pubmed/37540456 http://dx.doi.org/10.1007/s11033-023-08620-0 Text en © The Author(s) 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Mishima, Toshiaki Hosono, Kanako Tanabe, Mina Ito, Yoshiya Majima, Masataka Narumiya, Shuh Miyaji, Kagami Amano, Hideki Thromboxane prostanoid signaling in macrophages attenuates lymphedema and facilitates lymphangiogenesis in mice: TP signaling and lymphangiogenesis |
title | Thromboxane prostanoid signaling in macrophages attenuates lymphedema and facilitates lymphangiogenesis in mice: TP signaling and lymphangiogenesis |
title_full | Thromboxane prostanoid signaling in macrophages attenuates lymphedema and facilitates lymphangiogenesis in mice: TP signaling and lymphangiogenesis |
title_fullStr | Thromboxane prostanoid signaling in macrophages attenuates lymphedema and facilitates lymphangiogenesis in mice: TP signaling and lymphangiogenesis |
title_full_unstemmed | Thromboxane prostanoid signaling in macrophages attenuates lymphedema and facilitates lymphangiogenesis in mice: TP signaling and lymphangiogenesis |
title_short | Thromboxane prostanoid signaling in macrophages attenuates lymphedema and facilitates lymphangiogenesis in mice: TP signaling and lymphangiogenesis |
title_sort | thromboxane prostanoid signaling in macrophages attenuates lymphedema and facilitates lymphangiogenesis in mice: tp signaling and lymphangiogenesis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520203/ https://www.ncbi.nlm.nih.gov/pubmed/37540456 http://dx.doi.org/10.1007/s11033-023-08620-0 |
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