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Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae
Streptococcus agalactiae (S. agalactiae) is a contagious obligate parasite of the udder in dairy cows. Here, we examined S. agalactiae-host interactions in bovine mammary epithelial cells (BMECs) in vitro. We found that S. agalactiae infected BMECs through laminin β2 and integrin. Crk, Vps25, and Rh...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520442/ https://www.ncbi.nlm.nih.gov/pubmed/37766995 http://dx.doi.org/10.1016/j.isci.2023.107884 |
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author | Guo, Zhixin Ma, Yuze Jia, Zhibo Wang, Liping Lu, Xinyue Chen, Yuhao Wang, Yanfeng Hao, Huifang Yu, Shuixing Wang, Zhigang |
author_facet | Guo, Zhixin Ma, Yuze Jia, Zhibo Wang, Liping Lu, Xinyue Chen, Yuhao Wang, Yanfeng Hao, Huifang Yu, Shuixing Wang, Zhigang |
author_sort | Guo, Zhixin |
collection | PubMed |
description | Streptococcus agalactiae (S. agalactiae) is a contagious obligate parasite of the udder in dairy cows. Here, we examined S. agalactiae-host interactions in bovine mammary epithelial cells (BMECs) in vitro. We found that S. agalactiae infected BMECs through laminin β2 and integrin. Crk, Vps25, and RhoA were differentially expressed in S. agalactiae-infected cells. S. agalactiae infection activated FAK and Crk. FAK deficiency decreased the number of intracellular S. agalactiae and Crk activation. Knockdown of Crk or Vps25 increased the level of intracellular S. agalactiae, whereas its overexpression had the opposite effect. RhoA expression and actin cytoskeleton were altered in S. agalactiae-infected BMECs. Crk and Vps25 interact in cells, and invaded S. agalactiae also activates Crk, allowing it to cooperate with Vps25 to defend against intracellular infection by S. agalactiae. This study provides insights into the mechanism by which intracellular infection by S. agalactiae is regulated in BMECs. |
format | Online Article Text |
id | pubmed-10520442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105204422023-09-27 Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae Guo, Zhixin Ma, Yuze Jia, Zhibo Wang, Liping Lu, Xinyue Chen, Yuhao Wang, Yanfeng Hao, Huifang Yu, Shuixing Wang, Zhigang iScience Article Streptococcus agalactiae (S. agalactiae) is a contagious obligate parasite of the udder in dairy cows. Here, we examined S. agalactiae-host interactions in bovine mammary epithelial cells (BMECs) in vitro. We found that S. agalactiae infected BMECs through laminin β2 and integrin. Crk, Vps25, and RhoA were differentially expressed in S. agalactiae-infected cells. S. agalactiae infection activated FAK and Crk. FAK deficiency decreased the number of intracellular S. agalactiae and Crk activation. Knockdown of Crk or Vps25 increased the level of intracellular S. agalactiae, whereas its overexpression had the opposite effect. RhoA expression and actin cytoskeleton were altered in S. agalactiae-infected BMECs. Crk and Vps25 interact in cells, and invaded S. agalactiae also activates Crk, allowing it to cooperate with Vps25 to defend against intracellular infection by S. agalactiae. This study provides insights into the mechanism by which intracellular infection by S. agalactiae is regulated in BMECs. Elsevier 2023-09-09 /pmc/articles/PMC10520442/ /pubmed/37766995 http://dx.doi.org/10.1016/j.isci.2023.107884 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Guo, Zhixin Ma, Yuze Jia, Zhibo Wang, Liping Lu, Xinyue Chen, Yuhao Wang, Yanfeng Hao, Huifang Yu, Shuixing Wang, Zhigang Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae |
title | Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae |
title_full | Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae |
title_fullStr | Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae |
title_full_unstemmed | Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae |
title_short | Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae |
title_sort | crosstalk between integrin/fak and crk/vps25 governs invasion of bovine mammary epithelial cells by s. agalactiae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520442/ https://www.ncbi.nlm.nih.gov/pubmed/37766995 http://dx.doi.org/10.1016/j.isci.2023.107884 |
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