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A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films

Despite significant advancements in in vitro cardiac modeling approaches, researchers still lack the capacity to obtain in vitro measurements of a key indicator of cardiac function: contractility, or stroke volume under specific loading conditions—defined as the pressures to which the heart is subje...

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Autores principales: Yadid, Moran, Hagel, Mario, Labro, Megan Beldjilali, Le Roi, Baptiste, Flaxer, Carina, Flaxer, Eli, Barnea, A. Ronny, Tejman‐Yarden, Shai, Silberman, Eric, Li, Xin, Rauti, Rossana, Leichtmann‐Bardoogo, Yael, Yuan, Hongyan, Maoz, Ben M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520681/
https://www.ncbi.nlm.nih.gov/pubmed/37485582
http://dx.doi.org/10.1002/advs.202207498
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author Yadid, Moran
Hagel, Mario
Labro, Megan Beldjilali
Le Roi, Baptiste
Flaxer, Carina
Flaxer, Eli
Barnea, A. Ronny
Tejman‐Yarden, Shai
Silberman, Eric
Li, Xin
Rauti, Rossana
Leichtmann‐Bardoogo, Yael
Yuan, Hongyan
Maoz, Ben M.
author_facet Yadid, Moran
Hagel, Mario
Labro, Megan Beldjilali
Le Roi, Baptiste
Flaxer, Carina
Flaxer, Eli
Barnea, A. Ronny
Tejman‐Yarden, Shai
Silberman, Eric
Li, Xin
Rauti, Rossana
Leichtmann‐Bardoogo, Yael
Yuan, Hongyan
Maoz, Ben M.
author_sort Yadid, Moran
collection PubMed
description Despite significant advancements in in vitro cardiac modeling approaches, researchers still lack the capacity to obtain in vitro measurements of a key indicator of cardiac function: contractility, or stroke volume under specific loading conditions—defined as the pressures to which the heart is subjected prior to and during contraction. This work puts forward a platform that creates this capability, by providing a means of dynamically controlling loading conditions in vitro. This dynamic tissue loading platform consists of a thin magnetoresponsive hydrogel cantilever on which 2D engineered myocardial tissue is cultured. Exposing the cantilever to an external magnetic field—generated by positioning magnets at a controlled distance from the cantilever—causes the hydrogel film to stretch, creating tissue load. Next, cell contraction is induced through electrical stimulation, and the force of the contraction is recorded, by measuring the cantilever's deflection. Force–length‐based measurements of contractility are then derived, comparable to clinical measurements. In an illustrative application, the platform is used to measure contractility both in untreated myocardial tissue and in tissue exposed to an inotropic agent. Clear differences are observed between conditions, suggesting that the proposed platform has significant potential to provide clinically relevant measurements of contractility.
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spelling pubmed-105206812023-09-27 A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films Yadid, Moran Hagel, Mario Labro, Megan Beldjilali Le Roi, Baptiste Flaxer, Carina Flaxer, Eli Barnea, A. Ronny Tejman‐Yarden, Shai Silberman, Eric Li, Xin Rauti, Rossana Leichtmann‐Bardoogo, Yael Yuan, Hongyan Maoz, Ben M. Adv Sci (Weinh) Research Articles Despite significant advancements in in vitro cardiac modeling approaches, researchers still lack the capacity to obtain in vitro measurements of a key indicator of cardiac function: contractility, or stroke volume under specific loading conditions—defined as the pressures to which the heart is subjected prior to and during contraction. This work puts forward a platform that creates this capability, by providing a means of dynamically controlling loading conditions in vitro. This dynamic tissue loading platform consists of a thin magnetoresponsive hydrogel cantilever on which 2D engineered myocardial tissue is cultured. Exposing the cantilever to an external magnetic field—generated by positioning magnets at a controlled distance from the cantilever—causes the hydrogel film to stretch, creating tissue load. Next, cell contraction is induced through electrical stimulation, and the force of the contraction is recorded, by measuring the cantilever's deflection. Force–length‐based measurements of contractility are then derived, comparable to clinical measurements. In an illustrative application, the platform is used to measure contractility both in untreated myocardial tissue and in tissue exposed to an inotropic agent. Clear differences are observed between conditions, suggesting that the proposed platform has significant potential to provide clinically relevant measurements of contractility. John Wiley and Sons Inc. 2023-07-23 /pmc/articles/PMC10520681/ /pubmed/37485582 http://dx.doi.org/10.1002/advs.202207498 Text en © 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Yadid, Moran
Hagel, Mario
Labro, Megan Beldjilali
Le Roi, Baptiste
Flaxer, Carina
Flaxer, Eli
Barnea, A. Ronny
Tejman‐Yarden, Shai
Silberman, Eric
Li, Xin
Rauti, Rossana
Leichtmann‐Bardoogo, Yael
Yuan, Hongyan
Maoz, Ben M.
A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films
title A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films
title_full A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films
title_fullStr A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films
title_full_unstemmed A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films
title_short A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films
title_sort platform for assessing cellular contractile function based on magnetic manipulation of magnetoresponsive hydrogel films
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520681/
https://www.ncbi.nlm.nih.gov/pubmed/37485582
http://dx.doi.org/10.1002/advs.202207498
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