Cargando…

Melt‐Encoded‐Tags for Expanded Optical Readout in Digital PCR (METEOR‐dPCR) Enables Highly Multiplexed Quantitative Gene Panel Profiling

Digital PCR (dPCR) is an important tool for precise nucleic acid quantification in clinical setting, but the limited multiplexing capability restricts its applications for quantitative gene panel profiling. Here, this work describes melt‐encoded‐tags for expanded optical readout in digital PCR (METE...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Dong Dong, Muliaditan, Daniel, Viswanathan, Ramya, Cui, Xu, Cheow, Lih Feng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2023
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520687/ https://www.ncbi.nlm.nih.gov/pubmed/37485651 http://dx.doi.org/10.1002/advs.202301630

Ejemplares similares

qPCR, dPCR, NGS – A journey
por: Huggett, Jim F., et al.
Publicado: (2015)

dPCR: A Technology Review
por: Quan, Phenix-Lan, et al.
Publicado: (2018)

PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
por: Sidstedt, Maja, et al.
Publicado: (2020)

Diagnostic evaluation of qRT-PCR-based kit and dPCR-based kit for COVID-19
por: Lee, Cherl-Joon, et al.
Publicado: (2021)

Comparison among the Quantification of Bacterial Pathogens by qPCR, dPCR, and Cultural Methods
por: Ricchi, Matteo, et al.
Publicado: (2017)

Rapid and ultrasensitive digital PCR (dPCR) profiling of EGFRvIII in tumor cells and tissues
por: Saxena, Deeksha, et al.
Publicado: (2019)

Quantitative analysis of RNA by HPLC and evaluation of RT-dPCR for coronavirus RNA quantification
por: Niu, Chunyan, et al.
Publicado: (2021)

Massively parallel single-molecule telomere length measurement with digital real-time PCR
por: Luo, Yongqiang, et al.
Publicado: (2020)

Analytical Performance and Concordance with Next-Generation Sequencing of a Rapid, Multiplexed dPCR Panel for the Detection of DNA and RNA Biomarkers in Non-Small-Cell Lung Cancer
por: Cabrera, Kerri, et al.
Publicado: (2023)

Sequence specific sorting of DNA molecules with FACS using 3dPCR
por: Sukovich, David J., et al.
Publicado: (2017)

Temperature non-uniformity detection on dPCR chips and temperature sensor calibration
por: Gaňová, Martina, et al.
Publicado: (2022)

Heme Biosynthetic Gene Expression Analysis With dPCR in Erythropoietic Protoporphyria Patients
por: Granata, Francesca, et al.
Publicado: (2022)

A novel multiplex PCR for virus detection by melting curve analysis
por: Liu, Pengcheng, et al.
Publicado: (2018)

Increased sensitivity using real-time dPCR for detection of SARS-CoV-2
por: Duong, Kyra, et al.
Publicado: (2020)

Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events in Vivo
por: Falabella, Micol, et al.
Publicado: (2017)

Preliminary study of noninvasive prenatal screening for 22q11.2 deletion/duplication syndrome using multiplex dPCR assay
por: Wang, Jing, et al.
Publicado: (2023)

Image Segmentation and Quantification of Droplet dPCR Based on Thermal Bubble Printing Technology
por: Zhu, Mingjie, et al.
Publicado: (2022)

Vibrio-Sequins - dPCR-traceable DNA standards for quantitative genomics of Vibrio spp
por: Flütsch, Sabrina, et al.
Publicado: (2023)

“Stay in touch while on the bench” - how the MIQE applet can increase the quality of your qPCR and dPCR experiments
por: Abdel Nour, Afif M, et al.
Publicado: (2014)

Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater
por: Ahmed, Warish, et al.
Publicado: (2022)

Evaluation of saliva as an alternative diagnostic specimen source for SARS-CoV-2 detection by RT-dPCR
por: Sui, Zhiwei, et al.
Publicado: (2021)

Quantification of Actaea racemosa L. (black cohosh) from some of its potential adulterants using qPCR and dPCR methods
por: Shanmughanandhan, Jeevitha, et al.
Publicado: (2021)

529. Overdiagnosis of Clostridioides difficile with a Multiplex PCR Panel
por: Arora, Vaneet, et al.
Publicado: (2018)

338. The Use of Multiplex PCR Panel in the Diagnosis of Meningitis in Children
por: Taveras, Jeanette, et al.
Publicado: (2018)

Evaluation of process limit of detection and quantification variation of SARS-CoV-2 RT-qPCR and RT-dPCR assays for wastewater surveillance
por: Ahmed, Warish, et al.
Publicado: (2022)

Correlating qRT-PCR, dPCR and Viral Titration for the Identification and Quantification of SARS-CoV-2: A New Approach for Infection Management
por: Brandolini, Martina, et al.
Publicado: (2021)

Tracking plasma DNA mutation dynamics in estrogen receptor positive metastatic breast cancer with dPCR-SEQ
por: Kumar, Sunil, et al.
Publicado: (2018)

RT-dPCR in Mosquito Samples for ZIKV Detection: Effects of RNA Extraction and Reverse Transcription in Target Concentration
por: de Almeida, Paula Rodrigues, et al.
Publicado: (2020)

Single-channel multiplexing without melting curve analysis in real-time PCR
por: Lee, Young-Jo, et al.
Publicado: (2014)

Duplex dPCR System for Rapid Identification of Gram-Negative Pathogens in the Blood of Patients with Bloodstream Infection: A Culture-Independent Approach
por: Shin, Juyoun, et al.
Publicado: (2021)

Comparison of RT-dPCR and RT-qPCR and the effects of freeze–thaw cycle and glycine release buffer for wastewater SARS-CoV-2 analysis
por: Huge, Bonnie Jaskowski, et al.
Publicado: (2022)

874. Diagnostic Stewardship of Gastrointestinal Multiplex PCR Panels in Hospitalized Children
por: Cotter, Jillian, et al.
Publicado: (2022)

Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
por: Peixoto, Aline dos Santos, et al.
Publicado: (2020)

Introducing Meteor
por: Robinson, Josh, et al.
Publicado: (2015)

Meteor in action
por: Hochhaus, Stephan, et al.
Publicado: (2016)

Symposium on Meteors
por: Kaiser, T R
Publicado: (1955)

Les météores /
por: Margollé 1816-1884
Publicado: (1867)

Retinal meteor
por: Venkatesh, Ramesh, et al.
Publicado: (2017)

Development of multiplex microsatellite PCR panels for the seagrass Thalassia hemprichii (Hydrocharitaceae)(1)
por: van Dijk, Kor-jent, et al.
Publicado: (2014)

DNA Analysis by Restriction Enzyme (DARE) enables concurrent genomic and epigenomic characterization of single cells
por: Viswanathan, Ramya, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_6e0i00tqsbhgii293ih2lr2djd