An optimized protocol for chromatin immunoprecipitation from murine inguinal white adipose tissue

Chromatin immunoprecipitation (ChIP) protocols have been used to reveal protein-DNA interactions of various cell types and tissues; however, optimization is required for each specific type of sample. Here, we present a ChIP protocol from murine inguinal white adipose tissue. We describe steps for ti...

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Detalles Bibliográficos
Autores principales: Katsouda, Antonia, Valakos, Dimitrios, Vatsellas, Giannis, Thanos, Dimitris, Papapetropoulos, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520933/
https://www.ncbi.nlm.nih.gov/pubmed/37742172
http://dx.doi.org/10.1016/j.xpro.2023.102594
Descripción
Sumario:Chromatin immunoprecipitation (ChIP) protocols have been used to reveal protein-DNA interactions of various cell types and tissues; however, optimization is required for each specific type of sample. Here, we present a ChIP protocol from murine inguinal white adipose tissue. We describe steps for tissue harvesting, crosslinking, chromatin extraction, shearing, immunoprecipitation, and purification. We then detail procedures for analysis including library preparation, sequencing, and qRT-PCR validation. For complete details on the use and execution of this protocol, please refer to Antonia Katsouda et al. (2022).(1)

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