A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution

In this protocol, we describe a comparative approach to study the evolution of brain function in the Mexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca(2+) sensor GCaMP6s. We describe a methodology to prepar...

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Detalles Bibliográficos
Autores principales: Lloyd, Evan, Privat, Martin, Sumbre, German, Duboué, Erik R., Keene, Alex C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520939/
https://www.ncbi.nlm.nih.gov/pubmed/37742184
http://dx.doi.org/10.1016/j.xpro.2023.102517
Descripción
Sumario:In this protocol, we describe a comparative approach to study the evolution of brain function in the Mexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca(2+) sensor GCaMP6s. We describe a methodology to prepare samples and image activity across the optic tectum and olfactory bulb.

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