A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution

In this protocol, we describe a comparative approach to study the evolution of brain function in the Mexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca(2+) sensor GCaMP6s. We describe a methodology to prepar...

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Detalles Bibliográficos
Autores principales: Lloyd, Evan, Privat, Martin, Sumbre, German, Duboué, Erik R., Keene, Alex C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520939/
https://www.ncbi.nlm.nih.gov/pubmed/37742184
http://dx.doi.org/10.1016/j.xpro.2023.102517
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author Lloyd, Evan
Privat, Martin
Sumbre, German
Duboué, Erik R.
Keene, Alex C.
author_facet Lloyd, Evan
Privat, Martin
Sumbre, German
Duboué, Erik R.
Keene, Alex C.
author_sort Lloyd, Evan
collection PubMed
description In this protocol, we describe a comparative approach to study the evolution of brain function in the Mexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca(2+) sensor GCaMP6s. We describe a methodology to prepare samples and image activity across the optic tectum and olfactory bulb.
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spelling pubmed-105209392023-09-27 A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution Lloyd, Evan Privat, Martin Sumbre, German Duboué, Erik R. Keene, Alex C. STAR Protoc Protocol In this protocol, we describe a comparative approach to study the evolution of brain function in the Mexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca(2+) sensor GCaMP6s. We describe a methodology to prepare samples and image activity across the optic tectum and olfactory bulb. Elsevier 2023-09-23 /pmc/articles/PMC10520939/ /pubmed/37742184 http://dx.doi.org/10.1016/j.xpro.2023.102517 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lloyd, Evan
Privat, Martin
Sumbre, German
Duboué, Erik R.
Keene, Alex C.
A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution
title A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution
title_full A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution
title_fullStr A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution
title_full_unstemmed A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution
title_short A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution
title_sort protocol for whole-brain ca(2+) imaging in astyanax mexicanus, a model of comparative evolution
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520939/
https://www.ncbi.nlm.nih.gov/pubmed/37742184
http://dx.doi.org/10.1016/j.xpro.2023.102517
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