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A protocol for whole-brain Ca(2+) imaging in Astyanax mexicanus, a model of comparative evolution

In this protocol, we describe a comparative approach to study the evolution of brain function in the Mexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca(2+) sensor GCaMP6s. We describe a methodology to prepar...

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Detalles Bibliográficos
Autores principales:	Lloyd, Evan, Privat, Martin, Sumbre, German, Duboué, Erik R., Keene, Alex C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520939/ https://www.ncbi.nlm.nih.gov/pubmed/37742184 http://dx.doi.org/10.1016/j.xpro.2023.102517

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