Cargando…

Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative

[Image: see text] There is an urgent need for simple and non-invasive identification of live neural stem/progenitor cells (NSPCs) in the developing and adult brain as well as in disease, such as in brain tumors, due to the potential clinical importance in prognosis, diagnosis, and treatment of disea...

Descripción completa

Detalles Bibliográficos
Autores principales: Ilkhanizadeh, Shirin, Gracias, Aileen, Åslund, Andreas K.O., Bäck, Marcus, Simon, Rozalyn, Kavanagh, Edel, Migliori, Bianca, Neofytou, Christina, Nelander, Sven, Westermark, Bengt, Uhrbom, Lene, Forsberg-Nilsson, Karin, Konradsson, Peter, Teixeira, Ana I., Uhlén, Per, Joseph, Bertrand, Hermanson, Ola, Nilsson, K. Peter R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521023/
https://www.ncbi.nlm.nih.gov/pubmed/37647213
http://dx.doi.org/10.1021/acsabm.3c00447
_version_ 1785110051379216384
author Ilkhanizadeh, Shirin
Gracias, Aileen
Åslund, Andreas K.O.
Bäck, Marcus
Simon, Rozalyn
Kavanagh, Edel
Migliori, Bianca
Neofytou, Christina
Nelander, Sven
Westermark, Bengt
Uhrbom, Lene
Forsberg-Nilsson, Karin
Konradsson, Peter
Teixeira, Ana I.
Uhlén, Per
Joseph, Bertrand
Hermanson, Ola
Nilsson, K. Peter R.
author_facet Ilkhanizadeh, Shirin
Gracias, Aileen
Åslund, Andreas K.O.
Bäck, Marcus
Simon, Rozalyn
Kavanagh, Edel
Migliori, Bianca
Neofytou, Christina
Nelander, Sven
Westermark, Bengt
Uhrbom, Lene
Forsberg-Nilsson, Karin
Konradsson, Peter
Teixeira, Ana I.
Uhlén, Per
Joseph, Bertrand
Hermanson, Ola
Nilsson, K. Peter R.
author_sort Ilkhanizadeh, Shirin
collection PubMed
description [Image: see text] There is an urgent need for simple and non-invasive identification of live neural stem/progenitor cells (NSPCs) in the developing and adult brain as well as in disease, such as in brain tumors, due to the potential clinical importance in prognosis, diagnosis, and treatment of diseases of the nervous system. Here, we report a luminescent conjugated oligothiophene (LCO), named p-HTMI, for non-invasive and non-amplified real-time detection of live human patient-derived glioblastoma (GBM) stem cell-like cells and NSPCs. While p-HTMI stained only a small fraction of other cell types investigated, the mere addition of p-HTMI to the cell culture resulted in efficient detection of NSPCs or GBM cells from rodents and humans within minutes. p-HTMI is functionalized with a methylated imidazole moiety resembling the side chain of histidine/histamine, and non-methylated analogues were not functional. Cell sorting experiments of human GBM cells demonstrated that p-HTMI labeled the same cell population as CD271, a proposed marker for stem cell-like cells and rapidly migrating cells in glioblastoma. Our results suggest that the LCO p-HTMI is a versatile tool for immediate and selective detection of neural and glioma stem and progenitor cells.
format Online
Article
Text
id pubmed-10521023
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-105210232023-09-27 Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative Ilkhanizadeh, Shirin Gracias, Aileen Åslund, Andreas K.O. Bäck, Marcus Simon, Rozalyn Kavanagh, Edel Migliori, Bianca Neofytou, Christina Nelander, Sven Westermark, Bengt Uhrbom, Lene Forsberg-Nilsson, Karin Konradsson, Peter Teixeira, Ana I. Uhlén, Per Joseph, Bertrand Hermanson, Ola Nilsson, K. Peter R. ACS Appl Bio Mater [Image: see text] There is an urgent need for simple and non-invasive identification of live neural stem/progenitor cells (NSPCs) in the developing and adult brain as well as in disease, such as in brain tumors, due to the potential clinical importance in prognosis, diagnosis, and treatment of diseases of the nervous system. Here, we report a luminescent conjugated oligothiophene (LCO), named p-HTMI, for non-invasive and non-amplified real-time detection of live human patient-derived glioblastoma (GBM) stem cell-like cells and NSPCs. While p-HTMI stained only a small fraction of other cell types investigated, the mere addition of p-HTMI to the cell culture resulted in efficient detection of NSPCs or GBM cells from rodents and humans within minutes. p-HTMI is functionalized with a methylated imidazole moiety resembling the side chain of histidine/histamine, and non-methylated analogues were not functional. Cell sorting experiments of human GBM cells demonstrated that p-HTMI labeled the same cell population as CD271, a proposed marker for stem cell-like cells and rapidly migrating cells in glioblastoma. Our results suggest that the LCO p-HTMI is a versatile tool for immediate and selective detection of neural and glioma stem and progenitor cells. American Chemical Society 2023-08-30 /pmc/articles/PMC10521023/ /pubmed/37647213 http://dx.doi.org/10.1021/acsabm.3c00447 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Ilkhanizadeh, Shirin
Gracias, Aileen
Åslund, Andreas K.O.
Bäck, Marcus
Simon, Rozalyn
Kavanagh, Edel
Migliori, Bianca
Neofytou, Christina
Nelander, Sven
Westermark, Bengt
Uhrbom, Lene
Forsberg-Nilsson, Karin
Konradsson, Peter
Teixeira, Ana I.
Uhlén, Per
Joseph, Bertrand
Hermanson, Ola
Nilsson, K. Peter R.
Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative
title Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative
title_full Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative
title_fullStr Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative
title_full_unstemmed Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative
title_short Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative
title_sort live detection of neural progenitors and glioblastoma cells by an oligothiophene derivative
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521023/
https://www.ncbi.nlm.nih.gov/pubmed/37647213
http://dx.doi.org/10.1021/acsabm.3c00447
work_keys_str_mv AT ilkhanizadehshirin livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT graciasaileen livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT aslundandreasko livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT backmarcus livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT simonrozalyn livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT kavanaghedel livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT miglioribianca livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT neofytouchristina livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT nelandersven livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT westermarkbengt livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT uhrbomlene livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT forsbergnilssonkarin livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT konradssonpeter livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT teixeiraanai livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT uhlenper livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT josephbertrand livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT hermansonola livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative
AT nilssonkpeterr livedetectionofneuralprogenitorsandglioblastomacellsbyanoligothiophenederivative