Characterization of CoCas9 nuclease from Capnocytophaga ochracea

Cas9 nucleases are widely used for genome editing and engineering. Cas9 enzymes encoded by CRISPR-Cas defence systems of various prokaryotic organisms possess different properties such as target site preferences, size, and DNA cleavage efficiency. Here, we biochemically characterized CoCas9 from Cap...

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Autores principales: Vasileva, A., Selkova, P., Arseniev, A., Abramova, M., Shcheglova, N., Musharova, O., Mizgirev, I., Artamonova, T., Khodorkovskii, M., Severinov, K., Fedorova, I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521337/
https://www.ncbi.nlm.nih.gov/pubmed/37743659
http://dx.doi.org/10.1080/15476286.2023.2256578
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author Vasileva, A.
Selkova, P.
Arseniev, A.
Abramova, M.
Shcheglova, N.
Musharova, O.
Mizgirev, I.
Artamonova, T.
Khodorkovskii, M.
Severinov, K.
Fedorova, I.
author_facet Vasileva, A.
Selkova, P.
Arseniev, A.
Abramova, M.
Shcheglova, N.
Musharova, O.
Mizgirev, I.
Artamonova, T.
Khodorkovskii, M.
Severinov, K.
Fedorova, I.
author_sort Vasileva, A.
collection PubMed
description Cas9 nucleases are widely used for genome editing and engineering. Cas9 enzymes encoded by CRISPR-Cas defence systems of various prokaryotic organisms possess different properties such as target site preferences, size, and DNA cleavage efficiency. Here, we biochemically characterized CoCas9 from Capnocytophaga ochracea, a bacterium that inhabits the oral cavity of humans and contributes to plaque formation on teeth. CoCas9 recognizes a novel 5’-NRRWC-3’ PAM and efficiently cleaves DNA in vitro. Functional characterization of CoCas9 opens ways for genetic engineering of C. ochracea using its endogenous CRISPR-Cas system. The novel PAM requirement makes CoCas9 potentially useful in genome editing applications.
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spelling pubmed-105213372023-09-27 Characterization of CoCas9 nuclease from Capnocytophaga ochracea Vasileva, A. Selkova, P. Arseniev, A. Abramova, M. Shcheglova, N. Musharova, O. Mizgirev, I. Artamonova, T. Khodorkovskii, M. Severinov, K. Fedorova, I. RNA Biol Research Paper Cas9 nucleases are widely used for genome editing and engineering. Cas9 enzymes encoded by CRISPR-Cas defence systems of various prokaryotic organisms possess different properties such as target site preferences, size, and DNA cleavage efficiency. Here, we biochemically characterized CoCas9 from Capnocytophaga ochracea, a bacterium that inhabits the oral cavity of humans and contributes to plaque formation on teeth. CoCas9 recognizes a novel 5’-NRRWC-3’ PAM and efficiently cleaves DNA in vitro. Functional characterization of CoCas9 opens ways for genetic engineering of C. ochracea using its endogenous CRISPR-Cas system. The novel PAM requirement makes CoCas9 potentially useful in genome editing applications. Taylor & Francis 2023-09-24 /pmc/articles/PMC10521337/ /pubmed/37743659 http://dx.doi.org/10.1080/15476286.2023.2256578 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Research Paper
Vasileva, A.
Selkova, P.
Arseniev, A.
Abramova, M.
Shcheglova, N.
Musharova, O.
Mizgirev, I.
Artamonova, T.
Khodorkovskii, M.
Severinov, K.
Fedorova, I.
Characterization of CoCas9 nuclease from Capnocytophaga ochracea
title Characterization of CoCas9 nuclease from Capnocytophaga ochracea
title_full Characterization of CoCas9 nuclease from Capnocytophaga ochracea
title_fullStr Characterization of CoCas9 nuclease from Capnocytophaga ochracea
title_full_unstemmed Characterization of CoCas9 nuclease from Capnocytophaga ochracea
title_short Characterization of CoCas9 nuclease from Capnocytophaga ochracea
title_sort characterization of cocas9 nuclease from capnocytophaga ochracea
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521337/
https://www.ncbi.nlm.nih.gov/pubmed/37743659
http://dx.doi.org/10.1080/15476286.2023.2256578
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