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The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms
BACKGROUND: Drought resistance is a complex characteristic closely related to the severity and duration of stress. Perennial ryegrass (Lolium perenne L.) has no distinct drought tolerance but often encounters drought stress seasonally. Although the response of perennial ryegrass to either extreme or...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521523/ https://www.ncbi.nlm.nih.gov/pubmed/37749497 http://dx.doi.org/10.1186/s12870-023-04460-z |
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author | Wang, Dan Zhang, Yuting Chen, Chunyan Chen, Ruixin Bai, Xuechun Qiang, Zhiquan Fu, Juanjuan Qin, Tao |
author_facet | Wang, Dan Zhang, Yuting Chen, Chunyan Chen, Ruixin Bai, Xuechun Qiang, Zhiquan Fu, Juanjuan Qin, Tao |
author_sort | Wang, Dan |
collection | PubMed |
description | BACKGROUND: Drought resistance is a complex characteristic closely related to the severity and duration of stress. Perennial ryegrass (Lolium perenne L.) has no distinct drought tolerance but often encounters drought stress seasonally. Although the response of perennial ryegrass to either extreme or moderate drought stress has been investigated, a comprehensive understanding of perennial ryegrass response to both conditions of drought stress is currently lacking. RESULTS: In this study, we investigated the genetic variation in drought resistance in 18 perennial ryegrass varieties under both extreme and moderate drought conditions. The performance of these varieties exhibited obvious diversity, and the survival of perennial ryegrass under severe stress was not equal to good growth under moderate drought stress. ‘Sopin’, with superior performance under both stress conditions, was the best-performing variety. Transcriptome, physiological, and molecular analyses revealed that ‘Sopin’ adapted to drought stress through multiple sophisticated mechanisms. Under stress conditions, starch and sugar metabolic enzymes were highly expressed, while CslA was expressed at low levels in ‘Sopin’, promoting starch degradation and soluble sugar accumulation. The expression and activity of superoxide dismutase were significantly higher in ‘Sopin’, while the activity of peroxidase was lower, allowing for ‘Sopin’ to maintain a better balance between maintaining ROS signal transduction and alleviating oxidative damage. Furthermore, drought stress-related transcriptional and posttranscriptional regulatory mechanisms, including the upregulation of transcription factors, kinases, and E3 ubiquitin ligases, facilitate abscisic acid and stress signal transduction. CONCLUSION: Our study provides insights into the resistance of perennial ryegrass to both extreme and moderate droughts and the underlying mechanisms by which perennial ryegrass adapts to drought conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04460-z. |
format | Online Article Text |
id | pubmed-10521523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-105215232023-09-27 The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms Wang, Dan Zhang, Yuting Chen, Chunyan Chen, Ruixin Bai, Xuechun Qiang, Zhiquan Fu, Juanjuan Qin, Tao BMC Plant Biol Research BACKGROUND: Drought resistance is a complex characteristic closely related to the severity and duration of stress. Perennial ryegrass (Lolium perenne L.) has no distinct drought tolerance but often encounters drought stress seasonally. Although the response of perennial ryegrass to either extreme or moderate drought stress has been investigated, a comprehensive understanding of perennial ryegrass response to both conditions of drought stress is currently lacking. RESULTS: In this study, we investigated the genetic variation in drought resistance in 18 perennial ryegrass varieties under both extreme and moderate drought conditions. The performance of these varieties exhibited obvious diversity, and the survival of perennial ryegrass under severe stress was not equal to good growth under moderate drought stress. ‘Sopin’, with superior performance under both stress conditions, was the best-performing variety. Transcriptome, physiological, and molecular analyses revealed that ‘Sopin’ adapted to drought stress through multiple sophisticated mechanisms. Under stress conditions, starch and sugar metabolic enzymes were highly expressed, while CslA was expressed at low levels in ‘Sopin’, promoting starch degradation and soluble sugar accumulation. The expression and activity of superoxide dismutase were significantly higher in ‘Sopin’, while the activity of peroxidase was lower, allowing for ‘Sopin’ to maintain a better balance between maintaining ROS signal transduction and alleviating oxidative damage. Furthermore, drought stress-related transcriptional and posttranscriptional regulatory mechanisms, including the upregulation of transcription factors, kinases, and E3 ubiquitin ligases, facilitate abscisic acid and stress signal transduction. CONCLUSION: Our study provides insights into the resistance of perennial ryegrass to both extreme and moderate droughts and the underlying mechanisms by which perennial ryegrass adapts to drought conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04460-z. BioMed Central 2023-09-26 /pmc/articles/PMC10521523/ /pubmed/37749497 http://dx.doi.org/10.1186/s12870-023-04460-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Wang, Dan Zhang, Yuting Chen, Chunyan Chen, Ruixin Bai, Xuechun Qiang, Zhiquan Fu, Juanjuan Qin, Tao The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms |
title | The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms |
title_full | The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms |
title_fullStr | The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms |
title_full_unstemmed | The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms |
title_short | The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms |
title_sort | genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521523/ https://www.ncbi.nlm.nih.gov/pubmed/37749497 http://dx.doi.org/10.1186/s12870-023-04460-z |
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