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Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential

BACKGROUND: Toxigenic fungi (Aspergillus and Fusarium) and their metabolites represent the major cause of corn and corn-based products contamination and consequently lead to severe economic and health issues. AIM: Our current study aimed to investigate the efficacy of using L. macroides Bac6 as a bi...

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Autores principales: Mahmoud, Ahmed Lotfy E., Kilany, Ayat H. A. Mohamed, Hassan, Elhagag A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521556/
https://www.ncbi.nlm.nih.gov/pubmed/37752474
http://dx.doi.org/10.1186/s12866-023-03007-4
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author Mahmoud, Ahmed Lotfy E.
Kilany, Ayat H. A. Mohamed
Hassan, Elhagag A.
author_facet Mahmoud, Ahmed Lotfy E.
Kilany, Ayat H. A. Mohamed
Hassan, Elhagag A.
author_sort Mahmoud, Ahmed Lotfy E.
collection PubMed
description BACKGROUND: Toxigenic fungi (Aspergillus and Fusarium) and their metabolites represent the major cause of corn and corn-based products contamination and consequently lead to severe economic and health issues. AIM: Our current study aimed to investigate the efficacy of using L. macroides Bac6 as a biological control agent against the toxigenic fungi; A. flavus f10 and F. proliferatum f30 and their mycotoxins. RESULTS: The results illustrated that A. flavus f10 produced the aflatoxins AFB(1) and AFG(2) with concentrations of 21.239 and 13.593 ppb, respectively. While F. proliferatum f30 produced fumonisin B(1) (9600 ppb). Furthermore, L. macroides showed a high potential for inhibition of toxigenic fungal growth using a dual culture method. F. proliferatum f30 and A. flavus f10 were found to be inhibited by a percentage of 80 and 62.5%, respectively. The results were confirmed using the scanning electron microscope. The antagonistic bacteria, L. macroides, showed chitinase productivity and activity of 26.45 U/L and 0.12 U/mL/min, respectively, which illustrates its potential application as a biocontrol agent. The GC-MS analysis revealed an abundance of Pyrrolo[1,2-a] pyrazine-1,4-dione, Hexahydro in the bacterial supernatant that exhibited antifungal characteristics. L. macroides had a significant reduction of AFB(1) and AFG(2) produced by A. flavus f10, recording 99.25% and 99% inhibition, respectively. It also showed strong inhibition of fumonisin B(1) (90% inhibition) produced by F. proliferatum f30. Conclusion: Thus, the current study is a prospective study evaluating for the first time the potential impact of L. macroides Bac6 against the toxigenic fungi and their toxins. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-03007-4.
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spelling pubmed-105215562023-09-27 Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential Mahmoud, Ahmed Lotfy E. Kilany, Ayat H. A. Mohamed Hassan, Elhagag A. BMC Microbiol Research BACKGROUND: Toxigenic fungi (Aspergillus and Fusarium) and their metabolites represent the major cause of corn and corn-based products contamination and consequently lead to severe economic and health issues. AIM: Our current study aimed to investigate the efficacy of using L. macroides Bac6 as a biological control agent against the toxigenic fungi; A. flavus f10 and F. proliferatum f30 and their mycotoxins. RESULTS: The results illustrated that A. flavus f10 produced the aflatoxins AFB(1) and AFG(2) with concentrations of 21.239 and 13.593 ppb, respectively. While F. proliferatum f30 produced fumonisin B(1) (9600 ppb). Furthermore, L. macroides showed a high potential for inhibition of toxigenic fungal growth using a dual culture method. F. proliferatum f30 and A. flavus f10 were found to be inhibited by a percentage of 80 and 62.5%, respectively. The results were confirmed using the scanning electron microscope. The antagonistic bacteria, L. macroides, showed chitinase productivity and activity of 26.45 U/L and 0.12 U/mL/min, respectively, which illustrates its potential application as a biocontrol agent. The GC-MS analysis revealed an abundance of Pyrrolo[1,2-a] pyrazine-1,4-dione, Hexahydro in the bacterial supernatant that exhibited antifungal characteristics. L. macroides had a significant reduction of AFB(1) and AFG(2) produced by A. flavus f10, recording 99.25% and 99% inhibition, respectively. It also showed strong inhibition of fumonisin B(1) (90% inhibition) produced by F. proliferatum f30. Conclusion: Thus, the current study is a prospective study evaluating for the first time the potential impact of L. macroides Bac6 against the toxigenic fungi and their toxins. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-03007-4. BioMed Central 2023-09-26 /pmc/articles/PMC10521556/ /pubmed/37752474 http://dx.doi.org/10.1186/s12866-023-03007-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mahmoud, Ahmed Lotfy E.
Kilany, Ayat H. A. Mohamed
Hassan, Elhagag A.
Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential
title Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential
title_full Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential
title_fullStr Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential
title_full_unstemmed Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential
title_short Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential
title_sort antifungal activity of lysinibacillus macroides against toxigenic aspergillus flavus and fusarium proliferatum and analysis of its mycotoxin minimization potential
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521556/
https://www.ncbi.nlm.nih.gov/pubmed/37752474
http://dx.doi.org/10.1186/s12866-023-03007-4
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