Cargando…
Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives
Second harmonic generation (SHG) and third harmonic generation (THG) microscopies have emerged as powerful imaging modalities to examine structural properties of a wide range of biological tissues. Although SHG and THG arise from very different contrast mechanisms, the two are complimentary and can...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAAS
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521653/ https://www.ncbi.nlm.nih.gov/pubmed/37849910 http://dx.doi.org/10.34133/2021/3973857 |
_version_ | 1785110177318436864 |
---|---|
author | James, Darian S. Campagnola, Paul J. |
author_facet | James, Darian S. Campagnola, Paul J. |
author_sort | James, Darian S. |
collection | PubMed |
description | Second harmonic generation (SHG) and third harmonic generation (THG) microscopies have emerged as powerful imaging modalities to examine structural properties of a wide range of biological tissues. Although SHG and THG arise from very different contrast mechanisms, the two are complimentary and can often be collected simultaneously using a modified multiphoton microscope. In this review, we discuss the needed instrumentation for these modalities as well as the underlying theoretical principles of SHG and THG in tissue and describe how these can be leveraged to extract unique structural information. We provide an overview of recent advances showing how SHG microscopy has been used to evaluate collagen alterations in the extracellular matrix and how this has been used to advance our knowledge of cancers, fibroses, and the cornea, as well as in tissue engineering applications. Specific examples using polarization-resolved approaches and machine learning algorithms are highlighted. Similarly, we review how THG has enabled developmental biology and skin cancer studies due to its sensitivity to changes in refractive index, which are ubiquitous in all cell and tissue assemblies. Lastly, we offer perspectives and outlooks on future directions of SHG and THG microscopies and present unresolved questions, especially in terms of overall miniaturization and the development of microendoscopy instrumentation. |
format | Online Article Text |
id | pubmed-10521653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AAAS |
record_format | MEDLINE/PubMed |
spelling | pubmed-105216532023-10-17 Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives James, Darian S. Campagnola, Paul J. BME Front Review Article Second harmonic generation (SHG) and third harmonic generation (THG) microscopies have emerged as powerful imaging modalities to examine structural properties of a wide range of biological tissues. Although SHG and THG arise from very different contrast mechanisms, the two are complimentary and can often be collected simultaneously using a modified multiphoton microscope. In this review, we discuss the needed instrumentation for these modalities as well as the underlying theoretical principles of SHG and THG in tissue and describe how these can be leveraged to extract unique structural information. We provide an overview of recent advances showing how SHG microscopy has been used to evaluate collagen alterations in the extracellular matrix and how this has been used to advance our knowledge of cancers, fibroses, and the cornea, as well as in tissue engineering applications. Specific examples using polarization-resolved approaches and machine learning algorithms are highlighted. Similarly, we review how THG has enabled developmental biology and skin cancer studies due to its sensitivity to changes in refractive index, which are ubiquitous in all cell and tissue assemblies. Lastly, we offer perspectives and outlooks on future directions of SHG and THG microscopies and present unresolved questions, especially in terms of overall miniaturization and the development of microendoscopy instrumentation. AAAS 2021-01-25 /pmc/articles/PMC10521653/ /pubmed/37849910 http://dx.doi.org/10.34133/2021/3973857 Text en Copyright © 2021 Darian S. James and Paul J. Campagnola. https://creativecommons.org/licenses/by/4.0/Exclusive Licensee Suzhou Institute of Biomedical Engineering and Technology, CAS. Distributed under a Creative Commons Attribution License (CC BY 4.0). (https://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Review Article James, Darian S. Campagnola, Paul J. Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives |
title | Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives |
title_full | Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives |
title_fullStr | Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives |
title_full_unstemmed | Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives |
title_short | Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives |
title_sort | recent advancements in optical harmonic generation microscopy: applications and perspectives |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521653/ https://www.ncbi.nlm.nih.gov/pubmed/37849910 http://dx.doi.org/10.34133/2021/3973857 |
work_keys_str_mv | AT jamesdarians recentadvancementsinopticalharmonicgenerationmicroscopyapplicationsandperspectives AT campagnolapaulj recentadvancementsinopticalharmonicgenerationmicroscopyapplicationsandperspectives |