KIF18A improves migration and invasion of colorectal cancer (CRC) cells through inhibiting PTEN signaling

Background: Kinesin family member 18A (KIF18A) is involved in the development of a variety of human malignancies. However, we have never known the influences of KIF18A on colorectal cancer (CRC). The study is designed to investigate the effect and molecular mechanism of KIF18A on the progression of colorectal cancer. Methods: We have not only analyzed the database using GEO, but have examined the effect of KIF18A on the development of CRC by subcutaneous tumorigenesis in nude mice. HE staining was used to observe tumor size. Besides, we make use of Western blotting to monitor the expression of related proteins. In addition, the scratch wound assay and Transwell assay were conducted to detect the effect of KIF18A on the migration and invasion of CRC cells. Results: The results of GEO database analysis suggested that KIF18A had a positive correlation with the growth of CRC. The results of subcutaneous tumorigenesis and HE staining in nude mice explained that KIF18A promoted the progression of CRC. Both scratch wound assay and Transwell indicated that the migration and invasion of CRC could be promoted by KIF18A. The results of Western blot illustrated that KIF18A could forward the migration and invasion of CRC cells, and inhibit PTEN, which promoted the activation of PI3K/Akt signaling pathway, thus bringing about the expression of MMP2 and MMP9. Conclusion: In conclusion, KIF18A can further the activation of PI3K/Akt signaling pathway by means of inhibiting PTEN transcription. Therefore, it is inferred that that KIF18A is a therapeutic target for CRC.