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In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria

Soft rot disease affects a range of crops in the field and also during transit and storage, resulting in significant yield losses and negative economic impacts. This study evaluated the in vitro antibacterial activities and mode of action of Piper betle extracts against the soft rot disease-causing...

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Autores principales: Charirak, Punyisa, Prajantasan, Rapeepun, Premprayoon, Kantapon, Srikacha, Nikom, Ratananikom, Khakhanang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522424/
https://www.ncbi.nlm.nih.gov/pubmed/37766936
http://dx.doi.org/10.1155/2023/5806841
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author Charirak, Punyisa
Prajantasan, Rapeepun
Premprayoon, Kantapon
Srikacha, Nikom
Ratananikom, Khakhanang
author_facet Charirak, Punyisa
Prajantasan, Rapeepun
Premprayoon, Kantapon
Srikacha, Nikom
Ratananikom, Khakhanang
author_sort Charirak, Punyisa
collection PubMed
description Soft rot disease affects a range of crops in the field and also during transit and storage, resulting in significant yield losses and negative economic impacts. This study evaluated the in vitro antibacterial activities and mode of action of Piper betle extracts against the soft rot disease-causing bacteria, Erwinia caratovora subsp. caratovora (ECC). Dried leaves of P. betle were extracted with water, ethanol, and hexane solvents and evaluated for their antibacterial activity. The results showed the highest antibacterial activity against ECC in the ethanol extract, followed by hexane and water extracts with minimum inhibitory concentration (MIC) 1.562, 6.25, and more than 12.50 mg/mL, respectively. The time-kill assay indicated a bactericidal mode of action. ECC growth was destroyed within 6 and 8 hours after treatment with the ethanol extract at 4-fold MIC and 2-fold MIC, respectively. The ethanol extract of P. betle showed promising activity against ECC, with the potential for further development as a novel alternative treatment to control phytobacteria.
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spelling pubmed-105224242023-09-27 In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria Charirak, Punyisa Prajantasan, Rapeepun Premprayoon, Kantapon Srikacha, Nikom Ratananikom, Khakhanang Scientifica (Cairo) Research Article Soft rot disease affects a range of crops in the field and also during transit and storage, resulting in significant yield losses and negative economic impacts. This study evaluated the in vitro antibacterial activities and mode of action of Piper betle extracts against the soft rot disease-causing bacteria, Erwinia caratovora subsp. caratovora (ECC). Dried leaves of P. betle were extracted with water, ethanol, and hexane solvents and evaluated for their antibacterial activity. The results showed the highest antibacterial activity against ECC in the ethanol extract, followed by hexane and water extracts with minimum inhibitory concentration (MIC) 1.562, 6.25, and more than 12.50 mg/mL, respectively. The time-kill assay indicated a bactericidal mode of action. ECC growth was destroyed within 6 and 8 hours after treatment with the ethanol extract at 4-fold MIC and 2-fold MIC, respectively. The ethanol extract of P. betle showed promising activity against ECC, with the potential for further development as a novel alternative treatment to control phytobacteria. Hindawi 2023-09-19 /pmc/articles/PMC10522424/ /pubmed/37766936 http://dx.doi.org/10.1155/2023/5806841 Text en Copyright © 2023 Punyisa Charirak et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Charirak, Punyisa
Prajantasan, Rapeepun
Premprayoon, Kantapon
Srikacha, Nikom
Ratananikom, Khakhanang
In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria
title In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria
title_full In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria
title_fullStr In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria
title_full_unstemmed In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria
title_short In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria
title_sort in vitro antibacterial activity and mode of action of piper betle extracts against soft rot disease-causing bacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522424/
https://www.ncbi.nlm.nih.gov/pubmed/37766936
http://dx.doi.org/10.1155/2023/5806841
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