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Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration

Skin scarring devoid of dermal appendages after severe trauma has unfavorable effects on aesthetic and physiological functions. Here we present a method for large-area wound regeneration using biodegradable aligned extracellular matrix scaffolds. We show that the implantation of these scaffolds acce...

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Autores principales: Yang, Yang, Chu, Chenyu, Liu, Li, Wang, Chenbing, Hu, Chen, Rung, Shengan, Man, Yi, Qu, Yili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522601/
https://www.ncbi.nlm.nih.gov/pubmed/37752124
http://dx.doi.org/10.1038/s41467-023-41608-9
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author Yang, Yang
Chu, Chenyu
Liu, Li
Wang, Chenbing
Hu, Chen
Rung, Shengan
Man, Yi
Qu, Yili
author_facet Yang, Yang
Chu, Chenyu
Liu, Li
Wang, Chenbing
Hu, Chen
Rung, Shengan
Man, Yi
Qu, Yili
author_sort Yang, Yang
collection PubMed
description Skin scarring devoid of dermal appendages after severe trauma has unfavorable effects on aesthetic and physiological functions. Here we present a method for large-area wound regeneration using biodegradable aligned extracellular matrix scaffolds. We show that the implantation of these scaffolds accelerates wound coverage and enhances hair follicle neogenesis. We perform multimodal analysis, in combination with single-cell RNA sequencing and spatial transcriptomics, to explore the immune responses around biomaterials, highlighting the potential role of regulatory T cells in mitigating tissue fibrous by suppressing excessive type 2 inflammation. We find that immunodeficient mice lacking mature T lymphocytes show the typical characteristic of tissue fibrous driven by type 2 macrophage inflammation, validating the potential therapeutic effect of the adaptive immune system activated by biomaterials. These findings contribute to our understanding of the coordination of immune systems in wound regeneration and facilitate the design of immunoregulatory biomaterials in the future.
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spelling pubmed-105226012023-09-28 Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration Yang, Yang Chu, Chenyu Liu, Li Wang, Chenbing Hu, Chen Rung, Shengan Man, Yi Qu, Yili Nat Commun Article Skin scarring devoid of dermal appendages after severe trauma has unfavorable effects on aesthetic and physiological functions. Here we present a method for large-area wound regeneration using biodegradable aligned extracellular matrix scaffolds. We show that the implantation of these scaffolds accelerates wound coverage and enhances hair follicle neogenesis. We perform multimodal analysis, in combination with single-cell RNA sequencing and spatial transcriptomics, to explore the immune responses around biomaterials, highlighting the potential role of regulatory T cells in mitigating tissue fibrous by suppressing excessive type 2 inflammation. We find that immunodeficient mice lacking mature T lymphocytes show the typical characteristic of tissue fibrous driven by type 2 macrophage inflammation, validating the potential therapeutic effect of the adaptive immune system activated by biomaterials. These findings contribute to our understanding of the coordination of immune systems in wound regeneration and facilitate the design of immunoregulatory biomaterials in the future. Nature Publishing Group UK 2023-09-26 /pmc/articles/PMC10522601/ /pubmed/37752124 http://dx.doi.org/10.1038/s41467-023-41608-9 Text en © The Author(s) 2023, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yang, Yang
Chu, Chenyu
Liu, Li
Wang, Chenbing
Hu, Chen
Rung, Shengan
Man, Yi
Qu, Yili
Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration
title Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration
title_full Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration
title_fullStr Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration
title_full_unstemmed Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration
title_short Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration
title_sort tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522601/
https://www.ncbi.nlm.nih.gov/pubmed/37752124
http://dx.doi.org/10.1038/s41467-023-41608-9
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