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Rapid, high-sensitivity detection of biomolecules using dual-comb biosensing

Rapid, sensitive detection of biomolecules is important for biosensing of infectious pathogens as well as biomarkers and pollutants. For example, biosensing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still strongly required for the fight against coronavirus disease 2019 (COVI...

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Autores principales: Miyamura, Shogo, Oe, Ryo, Nakahara, Takuya, Koresawa, Hidenori, Okada, Shota, Taue, Shuji, Tokizane, Yu, Minamikawa, Takeo, Yano, Taka-Aki, Otsuka, Kunihiro, Sakane, Ayuko, Sasaki, Takuya, Yasutomo, Koji, Kajisa, Taira, Yasui, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522648/
https://www.ncbi.nlm.nih.gov/pubmed/37752134
http://dx.doi.org/10.1038/s41598-023-41436-3
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author Miyamura, Shogo
Oe, Ryo
Nakahara, Takuya
Koresawa, Hidenori
Okada, Shota
Taue, Shuji
Tokizane, Yu
Minamikawa, Takeo
Yano, Taka-Aki
Otsuka, Kunihiro
Sakane, Ayuko
Sasaki, Takuya
Yasutomo, Koji
Kajisa, Taira
Yasui, Takeshi
author_facet Miyamura, Shogo
Oe, Ryo
Nakahara, Takuya
Koresawa, Hidenori
Okada, Shota
Taue, Shuji
Tokizane, Yu
Minamikawa, Takeo
Yano, Taka-Aki
Otsuka, Kunihiro
Sakane, Ayuko
Sasaki, Takuya
Yasutomo, Koji
Kajisa, Taira
Yasui, Takeshi
author_sort Miyamura, Shogo
collection PubMed
description Rapid, sensitive detection of biomolecules is important for biosensing of infectious pathogens as well as biomarkers and pollutants. For example, biosensing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still strongly required for the fight against coronavirus disease 2019 (COVID-19) pandemic. Here, we aim to achieve the rapid and sensitive detection of SARS-CoV-2 nucleocapsid protein antigen by enhancing the performance of optical biosensing based on optical frequency combs (OFC). The virus-concentration-dependent optical spectrum shift produced by antigen–antibody interactions is transformed into a photonic radio-frequency (RF) shift by a frequency conversion between the optical and RF regions in the OFC, facilitating rapid and sensitive detection with well-established electrical frequency measurements. Furthermore, active-dummy temperature-drift compensation with a dual-comb configuration enables the very small change in the virus-concentration-dependent signal to be extracted from the large, variable background signal caused by temperature disturbance. The achieved performance of dual-comb biosensing will greatly enhance the applicability of biosensors to viruses, biomarkers, environmental hormones, and so on.
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spelling pubmed-105226482023-09-28 Rapid, high-sensitivity detection of biomolecules using dual-comb biosensing Miyamura, Shogo Oe, Ryo Nakahara, Takuya Koresawa, Hidenori Okada, Shota Taue, Shuji Tokizane, Yu Minamikawa, Takeo Yano, Taka-Aki Otsuka, Kunihiro Sakane, Ayuko Sasaki, Takuya Yasutomo, Koji Kajisa, Taira Yasui, Takeshi Sci Rep Article Rapid, sensitive detection of biomolecules is important for biosensing of infectious pathogens as well as biomarkers and pollutants. For example, biosensing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still strongly required for the fight against coronavirus disease 2019 (COVID-19) pandemic. Here, we aim to achieve the rapid and sensitive detection of SARS-CoV-2 nucleocapsid protein antigen by enhancing the performance of optical biosensing based on optical frequency combs (OFC). The virus-concentration-dependent optical spectrum shift produced by antigen–antibody interactions is transformed into a photonic radio-frequency (RF) shift by a frequency conversion between the optical and RF regions in the OFC, facilitating rapid and sensitive detection with well-established electrical frequency measurements. Furthermore, active-dummy temperature-drift compensation with a dual-comb configuration enables the very small change in the virus-concentration-dependent signal to be extracted from the large, variable background signal caused by temperature disturbance. The achieved performance of dual-comb biosensing will greatly enhance the applicability of biosensors to viruses, biomarkers, environmental hormones, and so on. Nature Publishing Group UK 2023-09-26 /pmc/articles/PMC10522648/ /pubmed/37752134 http://dx.doi.org/10.1038/s41598-023-41436-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Miyamura, Shogo
Oe, Ryo
Nakahara, Takuya
Koresawa, Hidenori
Okada, Shota
Taue, Shuji
Tokizane, Yu
Minamikawa, Takeo
Yano, Taka-Aki
Otsuka, Kunihiro
Sakane, Ayuko
Sasaki, Takuya
Yasutomo, Koji
Kajisa, Taira
Yasui, Takeshi
Rapid, high-sensitivity detection of biomolecules using dual-comb biosensing
title Rapid, high-sensitivity detection of biomolecules using dual-comb biosensing
title_full Rapid, high-sensitivity detection of biomolecules using dual-comb biosensing
title_fullStr Rapid, high-sensitivity detection of biomolecules using dual-comb biosensing
title_full_unstemmed Rapid, high-sensitivity detection of biomolecules using dual-comb biosensing
title_short Rapid, high-sensitivity detection of biomolecules using dual-comb biosensing
title_sort rapid, high-sensitivity detection of biomolecules using dual-comb biosensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522648/
https://www.ncbi.nlm.nih.gov/pubmed/37752134
http://dx.doi.org/10.1038/s41598-023-41436-3
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