Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats

Mitochondrial respirometry allows for the comprehensive study of oxygen consumption within the electron transport system in tissues. However, limited techniques exist for analyzing frozen or biobanked intestinal tissues. Here, we present a protocol to evaluate the respiratory function of mitochondri...

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Detalles Bibliográficos
Autores principales: Mu, Chunlong, Shearer, Jane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522993/
https://www.ncbi.nlm.nih.gov/pubmed/37742174
http://dx.doi.org/10.1016/j.xpro.2023.102560
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author Mu, Chunlong
Shearer, Jane
author_facet Mu, Chunlong
Shearer, Jane
author_sort Mu, Chunlong
collection PubMed
description Mitochondrial respirometry allows for the comprehensive study of oxygen consumption within the electron transport system in tissues. However, limited techniques exist for analyzing frozen or biobanked intestinal tissues. Here, we present a protocol to evaluate the respiratory function of mitochondria in colonic tissues after cryopreservation at −80°C. We describe steps for rat dissection, respirometry calibration, and tissue preparation. We then detail measurement of oxygen respiration and protein concentration. This protocol facilitates the retrospective analysis of mitochondrial respiration in frozen tissue.
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spelling pubmed-105229932023-09-28 Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats Mu, Chunlong Shearer, Jane STAR Protoc Protocol Mitochondrial respirometry allows for the comprehensive study of oxygen consumption within the electron transport system in tissues. However, limited techniques exist for analyzing frozen or biobanked intestinal tissues. Here, we present a protocol to evaluate the respiratory function of mitochondria in colonic tissues after cryopreservation at −80°C. We describe steps for rat dissection, respirometry calibration, and tissue preparation. We then detail measurement of oxygen respiration and protein concentration. This protocol facilitates the retrospective analysis of mitochondrial respiration in frozen tissue. Elsevier 2023-09-23 /pmc/articles/PMC10522993/ /pubmed/37742174 http://dx.doi.org/10.1016/j.xpro.2023.102560 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Mu, Chunlong
Shearer, Jane
Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats
title Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats
title_full Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats
title_fullStr Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats
title_full_unstemmed Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats
title_short Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats
title_sort protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522993/
https://www.ncbi.nlm.nih.gov/pubmed/37742174
http://dx.doi.org/10.1016/j.xpro.2023.102560
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