Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses

Spatial transcriptomics couples visual spatial markers with gene expression levels, but slide space and cost limit the number of samples that can be processed. Here, we present a protocol for mounting brains from multiple mice onto a single capture area of a spatial transcriptomics slide. We describ...

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Detalles Bibliográficos
Autores principales: Sei, Yoshitaka J., Chaumeil, Myriam M., Nakamura, Ken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523026/
https://www.ncbi.nlm.nih.gov/pubmed/37742175
http://dx.doi.org/10.1016/j.xpro.2023.102617
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author Sei, Yoshitaka J.
Chaumeil, Myriam M.
Nakamura, Ken
author_facet Sei, Yoshitaka J.
Chaumeil, Myriam M.
Nakamura, Ken
author_sort Sei, Yoshitaka J.
collection PubMed
description Spatial transcriptomics couples visual spatial markers with gene expression levels, but slide space and cost limit the number of samples that can be processed. Here, we present a protocol for mounting brains from multiple mice onto a single capture area of a spatial transcriptomics slide. We describe steps for conjoining frozen hippocampal sections from different brains into a single cryostat block, reducing the quantity of reagents required. This protocol is applicable to a range of existing spatial genomics platforms. For complete details on the use and execution of this protocol, please refer to Li et al. (2023).(1)
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spelling pubmed-105230262023-09-28 Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses Sei, Yoshitaka J. Chaumeil, Myriam M. Nakamura, Ken STAR Protoc Protocol Spatial transcriptomics couples visual spatial markers with gene expression levels, but slide space and cost limit the number of samples that can be processed. Here, we present a protocol for mounting brains from multiple mice onto a single capture area of a spatial transcriptomics slide. We describe steps for conjoining frozen hippocampal sections from different brains into a single cryostat block, reducing the quantity of reagents required. This protocol is applicable to a range of existing spatial genomics platforms. For complete details on the use and execution of this protocol, please refer to Li et al. (2023).(1) Elsevier 2023-09-23 /pmc/articles/PMC10523026/ /pubmed/37742175 http://dx.doi.org/10.1016/j.xpro.2023.102617 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Sei, Yoshitaka J.
Chaumeil, Myriam M.
Nakamura, Ken
Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses
title Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses
title_full Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses
title_fullStr Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses
title_full_unstemmed Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses
title_short Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses
title_sort protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523026/
https://www.ncbi.nlm.nih.gov/pubmed/37742175
http://dx.doi.org/10.1016/j.xpro.2023.102617
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