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Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography
The jellyfish Cassiopea largely cover their carbon demand via photosynthates produced by microalgal endosymbionts, but how holobiont morphology and tissue optical properties affect the light microclimate and symbiont photosynthesis in Cassiopea remain unexplored. Here, we use optical coherence tomog...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523073/ https://www.ncbi.nlm.nih.gov/pubmed/37752841 http://dx.doi.org/10.1098/rspb.2023.0127 |
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author | Lyndby, Niclas Heidelberg Murthy, Swathi Bessette, Sandrine Jakobsen, Sofie Lindegaard Meibom, Anders Kühl, Michael |
author_facet | Lyndby, Niclas Heidelberg Murthy, Swathi Bessette, Sandrine Jakobsen, Sofie Lindegaard Meibom, Anders Kühl, Michael |
author_sort | Lyndby, Niclas Heidelberg |
collection | PubMed |
description | The jellyfish Cassiopea largely cover their carbon demand via photosynthates produced by microalgal endosymbionts, but how holobiont morphology and tissue optical properties affect the light microclimate and symbiont photosynthesis in Cassiopea remain unexplored. Here, we use optical coherence tomography (OCT) to study the morphology of Cassiopea medusae at high spatial resolution. We include detailed 3D reconstructions of external micromorphology, and show the spatial distribution of endosymbionts and white granules in the bell tissue. Furthermore, we use OCT data to extract inherent optical properties from light-scattering white granules in Cassiopea, and show that granules enhance local light-availability for symbionts in close proximity. Individual granules had a scattering coefficient of µ(s) = 200–300 cm(−1), and scattering anisotropy factor of g = 0.7, while large tissue-regions filled with white granules had a lower µ(s) = 40–100 cm(−1), and g = 0.8–0.9. We combined OCT information with isotopic labelling experiments to investigate the effect of enhanced light-availability in whitish tissue regions. Endosymbionts located in whitish tissue exhibited significantly higher carbon fixation compared to symbionts in anastomosing tissue (i.e. tissue without light-scattering white granules). Our findings support previous suggestions that white granules in Cassiopea play an important role in the host modulation of the light-microenvironment. |
format | Online Article Text |
id | pubmed-10523073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-105230732023-09-28 Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography Lyndby, Niclas Heidelberg Murthy, Swathi Bessette, Sandrine Jakobsen, Sofie Lindegaard Meibom, Anders Kühl, Michael Proc Biol Sci Biological Applications The jellyfish Cassiopea largely cover their carbon demand via photosynthates produced by microalgal endosymbionts, but how holobiont morphology and tissue optical properties affect the light microclimate and symbiont photosynthesis in Cassiopea remain unexplored. Here, we use optical coherence tomography (OCT) to study the morphology of Cassiopea medusae at high spatial resolution. We include detailed 3D reconstructions of external micromorphology, and show the spatial distribution of endosymbionts and white granules in the bell tissue. Furthermore, we use OCT data to extract inherent optical properties from light-scattering white granules in Cassiopea, and show that granules enhance local light-availability for symbionts in close proximity. Individual granules had a scattering coefficient of µ(s) = 200–300 cm(−1), and scattering anisotropy factor of g = 0.7, while large tissue-regions filled with white granules had a lower µ(s) = 40–100 cm(−1), and g = 0.8–0.9. We combined OCT information with isotopic labelling experiments to investigate the effect of enhanced light-availability in whitish tissue regions. Endosymbionts located in whitish tissue exhibited significantly higher carbon fixation compared to symbionts in anastomosing tissue (i.e. tissue without light-scattering white granules). Our findings support previous suggestions that white granules in Cassiopea play an important role in the host modulation of the light-microenvironment. The Royal Society 2023-09-27 /pmc/articles/PMC10523073/ /pubmed/37752841 http://dx.doi.org/10.1098/rspb.2023.0127 Text en © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Biological Applications Lyndby, Niclas Heidelberg Murthy, Swathi Bessette, Sandrine Jakobsen, Sofie Lindegaard Meibom, Anders Kühl, Michael Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography |
title | Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography |
title_full | Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography |
title_fullStr | Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography |
title_full_unstemmed | Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography |
title_short | Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography |
title_sort | non-invasive investigation of the morphology and optical properties of the upside-down jellyfish cassiopea with optical coherence tomography |
topic | Biological Applications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523073/ https://www.ncbi.nlm.nih.gov/pubmed/37752841 http://dx.doi.org/10.1098/rspb.2023.0127 |
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