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Global transcriptome profiling reveals root- and leaf-specific responses of barley (Hordeum vulgare L.) to H(2)O(2)
In cereal crops, such as barley (Hordeum vulgare L.), the ability to appropriately respond to environmental cues is an important factor for yield stability and thus for agricultural production. Reactive oxygen species (ROS), such as hydrogen peroxide (H(2)O(2)), are key components of signal transduc...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523330/ https://www.ncbi.nlm.nih.gov/pubmed/37771486 http://dx.doi.org/10.3389/fpls.2023.1223778 |
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author | Bhattacharyya, Sabarna Giridhar, Maya Meier, Bastian Peiter, Edgar Vothknecht, Ute C. Chigri, Fatima |
author_facet | Bhattacharyya, Sabarna Giridhar, Maya Meier, Bastian Peiter, Edgar Vothknecht, Ute C. Chigri, Fatima |
author_sort | Bhattacharyya, Sabarna |
collection | PubMed |
description | In cereal crops, such as barley (Hordeum vulgare L.), the ability to appropriately respond to environmental cues is an important factor for yield stability and thus for agricultural production. Reactive oxygen species (ROS), such as hydrogen peroxide (H(2)O(2)), are key components of signal transduction cascades involved in plant adaptation to changing environmental conditions. H(2)O(2)-mediated stress responses include the modulation of expression of stress-responsive genes required to cope with different abiotic and biotic stresses. Despite its importance, knowledge of the effects of H(2)O(2) on the barley transcriptome is still scarce. In this study, we identified global transcriptomic changes induced after application of 10 mM H(2)O(2) to five-day-old barley plants. In total, 1883 and 1001 differentially expressed genes (DEGs) were identified in roots and leaves, respectively. Most of these DEGs were organ-specific, with only 209 DEGs commonly regulated and 37 counter-regulated between both plant parts. A GO term analysis further confirmed that different processes were affected in roots and leaves. It revealed that DEGs in leaves mostly comprised genes associated with hormone signaling, response to H(2)O(2) and abiotic stresses. This includes many transcriptions factors and small heat shock proteins. DEGs in roots mostly comprised genes linked to crucial aspects of H(2)O(2) catabolism and oxidant detoxification, glutathione metabolism, as well as cell wall modulation. These categories include many peroxidases and glutathione transferases. As with leaves, the H(2)O(2) response category in roots contains small heat shock proteins, however, mostly different members of this family were affected and they were all regulated in the opposite direction in the two plant parts. Validation of the expression of the selected commonly regulated DEGs by qRT-PCR was consistent with the RNA-seq data. The data obtained in this study provide an insight into the molecular mechanisms of oxidative stress responses in barley, which might also play a role upon other stresses that induce oxidative bursts. |
format | Online Article Text |
id | pubmed-10523330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105233302023-09-28 Global transcriptome profiling reveals root- and leaf-specific responses of barley (Hordeum vulgare L.) to H(2)O(2) Bhattacharyya, Sabarna Giridhar, Maya Meier, Bastian Peiter, Edgar Vothknecht, Ute C. Chigri, Fatima Front Plant Sci Plant Science In cereal crops, such as barley (Hordeum vulgare L.), the ability to appropriately respond to environmental cues is an important factor for yield stability and thus for agricultural production. Reactive oxygen species (ROS), such as hydrogen peroxide (H(2)O(2)), are key components of signal transduction cascades involved in plant adaptation to changing environmental conditions. H(2)O(2)-mediated stress responses include the modulation of expression of stress-responsive genes required to cope with different abiotic and biotic stresses. Despite its importance, knowledge of the effects of H(2)O(2) on the barley transcriptome is still scarce. In this study, we identified global transcriptomic changes induced after application of 10 mM H(2)O(2) to five-day-old barley plants. In total, 1883 and 1001 differentially expressed genes (DEGs) were identified in roots and leaves, respectively. Most of these DEGs were organ-specific, with only 209 DEGs commonly regulated and 37 counter-regulated between both plant parts. A GO term analysis further confirmed that different processes were affected in roots and leaves. It revealed that DEGs in leaves mostly comprised genes associated with hormone signaling, response to H(2)O(2) and abiotic stresses. This includes many transcriptions factors and small heat shock proteins. DEGs in roots mostly comprised genes linked to crucial aspects of H(2)O(2) catabolism and oxidant detoxification, glutathione metabolism, as well as cell wall modulation. These categories include many peroxidases and glutathione transferases. As with leaves, the H(2)O(2) response category in roots contains small heat shock proteins, however, mostly different members of this family were affected and they were all regulated in the opposite direction in the two plant parts. Validation of the expression of the selected commonly regulated DEGs by qRT-PCR was consistent with the RNA-seq data. The data obtained in this study provide an insight into the molecular mechanisms of oxidative stress responses in barley, which might also play a role upon other stresses that induce oxidative bursts. Frontiers Media S.A. 2023-09-12 /pmc/articles/PMC10523330/ /pubmed/37771486 http://dx.doi.org/10.3389/fpls.2023.1223778 Text en Copyright © 2023 Bhattacharyya, Giridhar, Meier, Peiter, Vothknecht and Chigri https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Bhattacharyya, Sabarna Giridhar, Maya Meier, Bastian Peiter, Edgar Vothknecht, Ute C. Chigri, Fatima Global transcriptome profiling reveals root- and leaf-specific responses of barley (Hordeum vulgare L.) to H(2)O(2) |
title | Global transcriptome profiling reveals root- and leaf-specific responses of barley (Hordeum vulgare L.) to H(2)O(2)
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title_full | Global transcriptome profiling reveals root- and leaf-specific responses of barley (Hordeum vulgare L.) to H(2)O(2)
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title_fullStr | Global transcriptome profiling reveals root- and leaf-specific responses of barley (Hordeum vulgare L.) to H(2)O(2)
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title_full_unstemmed | Global transcriptome profiling reveals root- and leaf-specific responses of barley (Hordeum vulgare L.) to H(2)O(2)
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title_short | Global transcriptome profiling reveals root- and leaf-specific responses of barley (Hordeum vulgare L.) to H(2)O(2)
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title_sort | global transcriptome profiling reveals root- and leaf-specific responses of barley (hordeum vulgare l.) to h(2)o(2) |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523330/ https://www.ncbi.nlm.nih.gov/pubmed/37771486 http://dx.doi.org/10.3389/fpls.2023.1223778 |
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