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HP6/Umbrea is dispensable for viability and fertility, suggesting essentiality of newly evolved genes is rare

The newly evolved gene Heterochromatin Protein 6 (HP6), which has been previously classified as essential, challenged the dogma that functions required for viability are only seen in genes with a long evolutionary history. Based on previous RNA-sequencing analysis in Drosophila germ cells, we asked...

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Detalles Bibliográficos
Autores principales: Grill, Sherilyn, Riley, Ashley, Selvaraj, Monica, Lehmann, Ruth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523450/
https://www.ncbi.nlm.nih.gov/pubmed/37725638
http://dx.doi.org/10.1073/pnas.2309478120
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author Grill, Sherilyn
Riley, Ashley
Selvaraj, Monica
Lehmann, Ruth
author_facet Grill, Sherilyn
Riley, Ashley
Selvaraj, Monica
Lehmann, Ruth
author_sort Grill, Sherilyn
collection PubMed
description The newly evolved gene Heterochromatin Protein 6 (HP6), which has been previously classified as essential, challenged the dogma that functions required for viability are only seen in genes with a long evolutionary history. Based on previous RNA-sequencing analysis in Drosophila germ cells, we asked whether HP6 might play a role in germline development. Surprisingly, we found that CRISPR-generated HP6 mutants are viable and fertile. Using previously generated mutants, we identified an independent lethal allele and an RNAi off-target effect that prevented accurate interpretation of HP6 essentiality. By reviewing existing data, we found that the vast majority of young genes that were previously classified as essential were indeed viable when tested with orthologous methods. Together, our data call into question the frequency with which newly evolved genes gain essential functions and suggest that using multiple independent genetic methods is essential when probing the functions of young genes.
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spelling pubmed-105234502023-09-28 HP6/Umbrea is dispensable for viability and fertility, suggesting essentiality of newly evolved genes is rare Grill, Sherilyn Riley, Ashley Selvaraj, Monica Lehmann, Ruth Proc Natl Acad Sci U S A Biological Sciences The newly evolved gene Heterochromatin Protein 6 (HP6), which has been previously classified as essential, challenged the dogma that functions required for viability are only seen in genes with a long evolutionary history. Based on previous RNA-sequencing analysis in Drosophila germ cells, we asked whether HP6 might play a role in germline development. Surprisingly, we found that CRISPR-generated HP6 mutants are viable and fertile. Using previously generated mutants, we identified an independent lethal allele and an RNAi off-target effect that prevented accurate interpretation of HP6 essentiality. By reviewing existing data, we found that the vast majority of young genes that were previously classified as essential were indeed viable when tested with orthologous methods. Together, our data call into question the frequency with which newly evolved genes gain essential functions and suggest that using multiple independent genetic methods is essential when probing the functions of young genes. National Academy of Sciences 2023-09-19 2023-09-26 /pmc/articles/PMC10523450/ /pubmed/37725638 http://dx.doi.org/10.1073/pnas.2309478120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Grill, Sherilyn
Riley, Ashley
Selvaraj, Monica
Lehmann, Ruth
HP6/Umbrea is dispensable for viability and fertility, suggesting essentiality of newly evolved genes is rare
title HP6/Umbrea is dispensable for viability and fertility, suggesting essentiality of newly evolved genes is rare
title_full HP6/Umbrea is dispensable for viability and fertility, suggesting essentiality of newly evolved genes is rare
title_fullStr HP6/Umbrea is dispensable for viability and fertility, suggesting essentiality of newly evolved genes is rare
title_full_unstemmed HP6/Umbrea is dispensable for viability and fertility, suggesting essentiality of newly evolved genes is rare
title_short HP6/Umbrea is dispensable for viability and fertility, suggesting essentiality of newly evolved genes is rare
title_sort hp6/umbrea is dispensable for viability and fertility, suggesting essentiality of newly evolved genes is rare
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523450/
https://www.ncbi.nlm.nih.gov/pubmed/37725638
http://dx.doi.org/10.1073/pnas.2309478120
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