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Examining Sleep Modulation by Drosophila Ellipsoid Body Neurons
Recent work in Drosophila has uncovered several neighboring classes of sleep-regulatory neurons within the central complex. However, the logic of connectivity and network motifs remains limited by the incomplete examination of relevant cell types. Using a recent genetic–anatomic classification of el...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523840/ https://www.ncbi.nlm.nih.gov/pubmed/37679041 http://dx.doi.org/10.1523/ENEURO.0281-23.2023 |
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author | Singh, Prabhjit Aleman, Abigail Omoto, Jaison Jiro Nguyen, Bao-Chau Kandimalla, Pratyush Hartenstein, Volker Donlea, Jeffrey M. |
author_facet | Singh, Prabhjit Aleman, Abigail Omoto, Jaison Jiro Nguyen, Bao-Chau Kandimalla, Pratyush Hartenstein, Volker Donlea, Jeffrey M. |
author_sort | Singh, Prabhjit |
collection | PubMed |
description | Recent work in Drosophila has uncovered several neighboring classes of sleep-regulatory neurons within the central complex. However, the logic of connectivity and network motifs remains limited by the incomplete examination of relevant cell types. Using a recent genetic–anatomic classification of ellipsoid body ring neurons, we conducted a thermogenetic screen in female flies to assess sleep/wake behavior and identified two wake-promoting drivers that label ER3d neurons and two sleep-promoting drivers that express in ER3m cells. We then used intersectional genetics to refine driver expression patterns. Activation of ER3d cells shortened sleep bouts, suggesting a key role in sleep maintenance. While sleep-promoting drivers from our mini-screen label overlapping ER3m neurons, intersectional strategies cannot rule out sleep regulatory roles for additional neurons in their expression patterns. Suppressing GABA synthesis in ER3m neurons prevents postinjury sleep, and GABAergic ER3d cells are required for thermogenetically induced wakefulness. Finally, we use an activity-dependent fluorescent reporter for putative synaptic contacts to embed these neurons within the known sleep-regulatory network. ER3m and ER3d neurons may receive connections from wake-active Helicon/ExR1 cells, and ER3m neurons likely inhibit ER3d neurons. Together, these data suggest a neural mechanism by which previously uncharacterized circuit elements stabilize sleep–wake states. |
format | Online Article Text |
id | pubmed-10523840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Society for Neuroscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-105238402023-09-28 Examining Sleep Modulation by Drosophila Ellipsoid Body Neurons Singh, Prabhjit Aleman, Abigail Omoto, Jaison Jiro Nguyen, Bao-Chau Kandimalla, Pratyush Hartenstein, Volker Donlea, Jeffrey M. eNeuro Research Article: New Research Recent work in Drosophila has uncovered several neighboring classes of sleep-regulatory neurons within the central complex. However, the logic of connectivity and network motifs remains limited by the incomplete examination of relevant cell types. Using a recent genetic–anatomic classification of ellipsoid body ring neurons, we conducted a thermogenetic screen in female flies to assess sleep/wake behavior and identified two wake-promoting drivers that label ER3d neurons and two sleep-promoting drivers that express in ER3m cells. We then used intersectional genetics to refine driver expression patterns. Activation of ER3d cells shortened sleep bouts, suggesting a key role in sleep maintenance. While sleep-promoting drivers from our mini-screen label overlapping ER3m neurons, intersectional strategies cannot rule out sleep regulatory roles for additional neurons in their expression patterns. Suppressing GABA synthesis in ER3m neurons prevents postinjury sleep, and GABAergic ER3d cells are required for thermogenetically induced wakefulness. Finally, we use an activity-dependent fluorescent reporter for putative synaptic contacts to embed these neurons within the known sleep-regulatory network. ER3m and ER3d neurons may receive connections from wake-active Helicon/ExR1 cells, and ER3m neurons likely inhibit ER3d neurons. Together, these data suggest a neural mechanism by which previously uncharacterized circuit elements stabilize sleep–wake states. Society for Neuroscience 2023-09-25 /pmc/articles/PMC10523840/ /pubmed/37679041 http://dx.doi.org/10.1523/ENEURO.0281-23.2023 Text en Copyright © 2023 Singh et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article: New Research Singh, Prabhjit Aleman, Abigail Omoto, Jaison Jiro Nguyen, Bao-Chau Kandimalla, Pratyush Hartenstein, Volker Donlea, Jeffrey M. Examining Sleep Modulation by Drosophila Ellipsoid Body Neurons |
title | Examining Sleep Modulation by Drosophila Ellipsoid Body Neurons |
title_full | Examining Sleep Modulation by Drosophila Ellipsoid Body Neurons |
title_fullStr | Examining Sleep Modulation by Drosophila Ellipsoid Body Neurons |
title_full_unstemmed | Examining Sleep Modulation by Drosophila Ellipsoid Body Neurons |
title_short | Examining Sleep Modulation by Drosophila Ellipsoid Body Neurons |
title_sort | examining sleep modulation by drosophila ellipsoid body neurons |
topic | Research Article: New Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523840/ https://www.ncbi.nlm.nih.gov/pubmed/37679041 http://dx.doi.org/10.1523/ENEURO.0281-23.2023 |
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