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Identification of CD44 as a Reliable Biomarker for Glioblastoma Invasion: Based on Magnetic Resonance Imaging and Spectroscopic Analysis of 5-Aminolevulinic Acid Fluorescence
Recurrent glioblastoma multiforme (GBM) is largely attributed to peritumoral infiltration of tumor cells. As higher CD44 expression in the tumor periphery correlates with higher risk of GBM invasion, the present study analyzed the relationship between CD44 expression and magnetic resonance imaging (...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525185/ https://www.ncbi.nlm.nih.gov/pubmed/37760811 http://dx.doi.org/10.3390/biomedicines11092369 |
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author | Inoue, Akihiro Ohnishi, Takanori Nishikawa, Masahiro Watanabe, Hideaki Kusakabe, Kosuke Taniwaki, Mashio Yano, Hajime Ohtsuka, Yoshihiro Matsumoto, Shirabe Suehiro, Satoshi Yamashita, Daisuke Shigekawa, Seiji Takahashi, Hisaaki Kitazawa, Riko Tanaka, Junya Kunieda, Takeharu |
author_facet | Inoue, Akihiro Ohnishi, Takanori Nishikawa, Masahiro Watanabe, Hideaki Kusakabe, Kosuke Taniwaki, Mashio Yano, Hajime Ohtsuka, Yoshihiro Matsumoto, Shirabe Suehiro, Satoshi Yamashita, Daisuke Shigekawa, Seiji Takahashi, Hisaaki Kitazawa, Riko Tanaka, Junya Kunieda, Takeharu |
author_sort | Inoue, Akihiro |
collection | PubMed |
description | Recurrent glioblastoma multiforme (GBM) is largely attributed to peritumoral infiltration of tumor cells. As higher CD44 expression in the tumor periphery correlates with higher risk of GBM invasion, the present study analyzed the relationship between CD44 expression and magnetic resonance imaging (MRI)-based invasiveness of GBM on a large scale. We also quantitatively evaluated GBM invasion using 5-aminolevulinic acid (5-ALA) spectroscopy to investigate the relationship between CD44 expression and tumor invasiveness as evaluated by intraoperative 5-ALA intensity. Based on MRI, GBM was classified as high-invasive type in 28 patients and low-invasive type in 22 patients. High-invasive type expressed CD44 at a significantly higher level than low-invasive type and was associated with worse survival. To quantitatively analyze GBM invasiveness, the relationship between tumor density in the peritumoral area and the spectroscopic intensity of 5-ALA was investigated. Spectroscopy showed that the 5-ALA intensity of infiltrating tumor cells correlated with tumor density as represented by the Ki-67 staining index. No significant correlation between CD44 and degree of 5-ALA-based invasiveness of GBM was found, but invasiveness of GBM as evaluated by 5-ALA matched the classification from MRI in all except one case, indicating that CD44 expression at the GBM periphery could provide a reliable biomarker for invasiveness in GBM. |
format | Online Article Text |
id | pubmed-10525185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105251852023-09-28 Identification of CD44 as a Reliable Biomarker for Glioblastoma Invasion: Based on Magnetic Resonance Imaging and Spectroscopic Analysis of 5-Aminolevulinic Acid Fluorescence Inoue, Akihiro Ohnishi, Takanori Nishikawa, Masahiro Watanabe, Hideaki Kusakabe, Kosuke Taniwaki, Mashio Yano, Hajime Ohtsuka, Yoshihiro Matsumoto, Shirabe Suehiro, Satoshi Yamashita, Daisuke Shigekawa, Seiji Takahashi, Hisaaki Kitazawa, Riko Tanaka, Junya Kunieda, Takeharu Biomedicines Article Recurrent glioblastoma multiforme (GBM) is largely attributed to peritumoral infiltration of tumor cells. As higher CD44 expression in the tumor periphery correlates with higher risk of GBM invasion, the present study analyzed the relationship between CD44 expression and magnetic resonance imaging (MRI)-based invasiveness of GBM on a large scale. We also quantitatively evaluated GBM invasion using 5-aminolevulinic acid (5-ALA) spectroscopy to investigate the relationship between CD44 expression and tumor invasiveness as evaluated by intraoperative 5-ALA intensity. Based on MRI, GBM was classified as high-invasive type in 28 patients and low-invasive type in 22 patients. High-invasive type expressed CD44 at a significantly higher level than low-invasive type and was associated with worse survival. To quantitatively analyze GBM invasiveness, the relationship between tumor density in the peritumoral area and the spectroscopic intensity of 5-ALA was investigated. Spectroscopy showed that the 5-ALA intensity of infiltrating tumor cells correlated with tumor density as represented by the Ki-67 staining index. No significant correlation between CD44 and degree of 5-ALA-based invasiveness of GBM was found, but invasiveness of GBM as evaluated by 5-ALA matched the classification from MRI in all except one case, indicating that CD44 expression at the GBM periphery could provide a reliable biomarker for invasiveness in GBM. MDPI 2023-08-24 /pmc/articles/PMC10525185/ /pubmed/37760811 http://dx.doi.org/10.3390/biomedicines11092369 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Inoue, Akihiro Ohnishi, Takanori Nishikawa, Masahiro Watanabe, Hideaki Kusakabe, Kosuke Taniwaki, Mashio Yano, Hajime Ohtsuka, Yoshihiro Matsumoto, Shirabe Suehiro, Satoshi Yamashita, Daisuke Shigekawa, Seiji Takahashi, Hisaaki Kitazawa, Riko Tanaka, Junya Kunieda, Takeharu Identification of CD44 as a Reliable Biomarker for Glioblastoma Invasion: Based on Magnetic Resonance Imaging and Spectroscopic Analysis of 5-Aminolevulinic Acid Fluorescence |
title | Identification of CD44 as a Reliable Biomarker for Glioblastoma Invasion: Based on Magnetic Resonance Imaging and Spectroscopic Analysis of 5-Aminolevulinic Acid Fluorescence |
title_full | Identification of CD44 as a Reliable Biomarker for Glioblastoma Invasion: Based on Magnetic Resonance Imaging and Spectroscopic Analysis of 5-Aminolevulinic Acid Fluorescence |
title_fullStr | Identification of CD44 as a Reliable Biomarker for Glioblastoma Invasion: Based on Magnetic Resonance Imaging and Spectroscopic Analysis of 5-Aminolevulinic Acid Fluorescence |
title_full_unstemmed | Identification of CD44 as a Reliable Biomarker for Glioblastoma Invasion: Based on Magnetic Resonance Imaging and Spectroscopic Analysis of 5-Aminolevulinic Acid Fluorescence |
title_short | Identification of CD44 as a Reliable Biomarker for Glioblastoma Invasion: Based on Magnetic Resonance Imaging and Spectroscopic Analysis of 5-Aminolevulinic Acid Fluorescence |
title_sort | identification of cd44 as a reliable biomarker for glioblastoma invasion: based on magnetic resonance imaging and spectroscopic analysis of 5-aminolevulinic acid fluorescence |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525185/ https://www.ncbi.nlm.nih.gov/pubmed/37760811 http://dx.doi.org/10.3390/biomedicines11092369 |
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