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Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C

SIMPLE SUMMARY: Diluent plays an important role in sperm storage. The various components of the diluent provide nutrition for sperm survival, maintain the stability of the preserved environment, and prolong the survival time of sperm. However, for the preservation of semen at 4 °C, diluent formulati...

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Autores principales: Zhang, Liuming, Wang, Yanhu, Sun, Xiaomei, Kang, Yan, Sohail, Tariq, Wang, Jian, Li, Yongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525221/
https://www.ncbi.nlm.nih.gov/pubmed/37760223
http://dx.doi.org/10.3390/ani13182823
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author Zhang, Liuming
Wang, Yanhu
Sun, Xiaomei
Kang, Yan
Sohail, Tariq
Wang, Jian
Li, Yongjun
author_facet Zhang, Liuming
Wang, Yanhu
Sun, Xiaomei
Kang, Yan
Sohail, Tariq
Wang, Jian
Li, Yongjun
author_sort Zhang, Liuming
collection PubMed
description SIMPLE SUMMARY: Diluent plays an important role in sperm storage. The various components of the diluent provide nutrition for sperm survival, maintain the stability of the preserved environment, and prolong the survival time of sperm. However, for the preservation of semen at 4 °C, diluent formulations are currently lacking or existing diluent formulations are not sufficient. Therefore, the effect of four diluents on semen preservation was studied through an assessment of sperm motility and functional integrity. Diluent D (Tris–Fructose–Citric acid–Egg yolk) was found to greatly improve semen quality during storage at 4 °C. ABSTRACT: This study aimed to investigate the effects of various diluents on the quality of Hu ram sperm stored at 4 °C. Semen samples were collected from three Hu rams and diluted with diluents A (Sodium citrate–Glucose–Egg yolk), B (Sodium citrate–Glucose), C (Fructose–Skimmed milk powder–Soy lecithin), and D (Tris–Fructose–Citric acid–Egg yolk). Total motility (TM), straight-line velocity (VSL), average path velocity (VAP), curvilinear velocity (VCL), average motion degree (MAD), acrosome integrity, membrane integrity, and reactive oxygen species (ROS) were evaluated. The results showed that diluent D had better preservation in terms of the sperm TM, VSL, VCL, VAP, MAD, and membrane and acrosome integrity. On the third day of the storage, the sperm PM of diluent D was higher than that of other diluents (p < 0.05). The ROS level of diluent D was lower than that of other diluents on the fifth day (p < 0.05). On the seventh day of the storage, the sperm TM in diluent D reached 50%, which was the highest in all diluent groups. On the seventh day of the storage, the integrity of the sperm membrane and the integrity of the acrosome of the sperm in diluent D were the highest in all diluent groups (p < 0.05). In conclusion, these results indicated that diluent D improved the semen quality during storage at 4 °C. In this study, diluent D was the best diluent formula for Hu ram semen stored at 4 °C.
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spelling pubmed-105252212023-09-28 Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C Zhang, Liuming Wang, Yanhu Sun, Xiaomei Kang, Yan Sohail, Tariq Wang, Jian Li, Yongjun Animals (Basel) Article SIMPLE SUMMARY: Diluent plays an important role in sperm storage. The various components of the diluent provide nutrition for sperm survival, maintain the stability of the preserved environment, and prolong the survival time of sperm. However, for the preservation of semen at 4 °C, diluent formulations are currently lacking or existing diluent formulations are not sufficient. Therefore, the effect of four diluents on semen preservation was studied through an assessment of sperm motility and functional integrity. Diluent D (Tris–Fructose–Citric acid–Egg yolk) was found to greatly improve semen quality during storage at 4 °C. ABSTRACT: This study aimed to investigate the effects of various diluents on the quality of Hu ram sperm stored at 4 °C. Semen samples were collected from three Hu rams and diluted with diluents A (Sodium citrate–Glucose–Egg yolk), B (Sodium citrate–Glucose), C (Fructose–Skimmed milk powder–Soy lecithin), and D (Tris–Fructose–Citric acid–Egg yolk). Total motility (TM), straight-line velocity (VSL), average path velocity (VAP), curvilinear velocity (VCL), average motion degree (MAD), acrosome integrity, membrane integrity, and reactive oxygen species (ROS) were evaluated. The results showed that diluent D had better preservation in terms of the sperm TM, VSL, VCL, VAP, MAD, and membrane and acrosome integrity. On the third day of the storage, the sperm PM of diluent D was higher than that of other diluents (p < 0.05). The ROS level of diluent D was lower than that of other diluents on the fifth day (p < 0.05). On the seventh day of the storage, the sperm TM in diluent D reached 50%, which was the highest in all diluent groups. On the seventh day of the storage, the integrity of the sperm membrane and the integrity of the acrosome of the sperm in diluent D were the highest in all diluent groups (p < 0.05). In conclusion, these results indicated that diluent D improved the semen quality during storage at 4 °C. In this study, diluent D was the best diluent formula for Hu ram semen stored at 4 °C. MDPI 2023-09-06 /pmc/articles/PMC10525221/ /pubmed/37760223 http://dx.doi.org/10.3390/ani13182823 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Liuming
Wang, Yanhu
Sun, Xiaomei
Kang, Yan
Sohail, Tariq
Wang, Jian
Li, Yongjun
Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C
title Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C
title_full Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C
title_fullStr Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C
title_full_unstemmed Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C
title_short Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C
title_sort effects of different diluents on semen quality of hu ram stored at 4 °c
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525221/
https://www.ncbi.nlm.nih.gov/pubmed/37760223
http://dx.doi.org/10.3390/ani13182823
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